Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Connors, W.M.; Stotz, E.
    The purification and properties of a triacetic acid-hydrolyzing enzyme (1949), J. Biol. Chem., 178, 881-890.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.2 Benzoylacetone 9 mM Bos taurus
3.7.1.2 citrate 9 mM Bos taurus
3.7.1.2 Fe3+ inactivation by precipitating the proteins Bos taurus
3.7.1.2 La3+ inactivation by precipitating the proteins Bos taurus
3.7.1.2 malonate 9 mM Bos taurus
3.7.1.2 oxalate 9 mM Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.2 1.38
-
 triacetic acid
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.7.1.2 Cu2+
-
 Bos taurus
3.7.1.2 Mg2+
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.2 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.7.1.2 liver
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
3.7.1.2 4°C, 1 month Bos taurus
3.7.1.2 frozen, 3 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.2 2,4-diketohexanoate + H2O
-
 Bos taurus pyruvate + propanoate
-
 ?
3.7.1.2 triacetic acid + H2O
-
 Bos taurus acetoacetic acid + acetic acid
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.2 6.5
-
 in maleate buffer Bos taurus
3.7.1.2 8
-
 in phosphate and borate buffer Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.7.1.2 6 8.5
-
 Bos taurus