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Literature summary extracted from

  • Maley, G.F.; MacColl, R.; Maley, F.
    T2r+ bacteriophage-induced enzymes. II. The subunit structure of deoxycytidylate deaminase (1972), J. Biol. Chem., 247, 940-945.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.12 5-hydroxymethyl-dCTP natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP Escherichia phage T2
3.5.4.12 5-hydroxymethyl-dCTP pH-dependent activation Escherichia phage T2
3.5.4.12 dCTP pH-dependent activation Gallus gallus
3.5.4.12 dCTP positive allosteric effector Gallus gallus
3.5.4.12 dCTP positive allosteric effector Escherichia phage T2
3.5.4.12 dCTP activation by dCTP decreases markedly as the pH is increased, pH-dependent activation Escherichia phage T2

General Stability

EC Number General Stability Organism
3.5.4.12 2-mercaptoethanol stabilizes Escherichia phage T2
3.5.4.12 guanidine hydrochloride, 1 M, complete loss in activity accompanied by disaggregation of enzyme, 6 M: denaturation, complete reactivation with 50 mM 2-mercaptoethanol Escherichia phage T2

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.12 dTTP allosteric inhibitor Escherichia phage T2
3.5.4.12 dTTP allosteric inhibitor Gallus gallus
3.5.4.12 guanidine hydrochloride 0.5 M: 50% inhibition, 1 M: 100% inhibition Escherichia phage T2

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.12 20200
-
 6 * 20200, sedimentation in 6 M guanidine hydrochloride containing 0.1 M 2-mercaptoethanol and SDS-PAGE Escherichia phage T2
3.5.4.12 124000
-
 PAGE and sedimentation equilibrium Escherichia phage T2

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.12 dCMP + H2O Gallus gallus
-
 dUMP + NH3
-
 ?
3.5.4.12 dCMP + H2O Escherichia phage T2
-
 dUMP + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.12 Escherichia phage T2
-
 T2r+-infected Escherichia coli B
-
3.5.4.12 Gallus gallus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.4.12 dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Gallus gallus
a
3.5.4.12 dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Escherichia phage T2
a
3.5.4.12 dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Gallus gallus
a
3.5.4.12 dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Escherichia phage T2
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.4.12 after treatment with guanidine hydrochloride, 6 M, complete reactivation with 50 mM 2-mercaptoethanol, original hexameric structure restored Escherichia phage T2

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.12 embryo
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.12 dCMP + H2O
-
 Gallus gallus dUMP + NH3
-
 ?
3.5.4.12 dCMP + H2O
-
 Escherichia phage T2 dUMP + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.12 hexamer 6 identical subunits Gallus gallus
3.5.4.12 hexamer 6 identical subunits Escherichia phage T2
3.5.4.12 hexamer hexamer is the active form Escherichia phage T2
3.5.4.12 hexamer 6 * 20200, sedimentation in 6 M guanidine hydrochloride containing 0.1 M 2-mercaptoethanol and SDS-PAGE Escherichia phage T2