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Literature summary extracted from

  • Rolton, H.A.; Keir, H.M.
    Deoxycytidylate deaminase. Evidence for a new enzyme in cells infected by the virus of Herpes simplex (1974), Biochem. J., 143, 403-409.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.12 dCTP enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster: maximum activation is achieved by lower concentration of dCTP than of enzyme from non-infected cells Herpes simplex virus
3.5.4.12 dCTP enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster: maximum activation is achieved by lower concentration of dCTP than of enzyme from non-infected cells Cricetinae
3.5.4.12 Herpes antiserum activation of host-cell enzyme Cricetinae
3.5.4.12 additional information no activation by KCl up to 0.3 M Herpes simplex virus
3.5.4.12 additional information no activation by KCl up to 0.3 M Cricetinae
3.5.4.12 pre-immune serum activation of host-cell enzyme, twice as effective as Herpes antiserum Cricetinae

General Stability

EC Number General Stability Organism
3.5.4.12 dCTP and Mg2+ stabilize Cricetinae
3.5.4.12 enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilize against heat inactivation Herpes simplex virus
3.5.4.12 enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilize against heat inactivation Cricetinae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.12 dTTP enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to inhibition by dTTP than enzyme from non-infected cells Cricetinae
3.5.4.12 dTTP enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to inhibition by dTTP than enzyme from non-infected cells Herpes simplex virus
3.5.4.12 Herpes antiserum inhibition of Herpes infected-cell enzyme Herpes simplex virus
3.5.4.12 additional information no inhibition by KCl up to 0.3 M Cricetinae
3.5.4.12 additional information no inhibition by KCl up to 0.3 M Herpes simplex virus
3.5.4.12 pre-immune serum inhibition of Herpes infected-cell enzyme Herpes simplex virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.12 0.32
-
dCMP in the presence of dCTP and presence of 2 mM Mg2+ Herpes simplex virus
3.5.4.12 0.32
-
dCMP in the presence of dCTP and presence of 2 mM Mg2+ Cricetinae
3.5.4.12 0.91
-
dCMP in the presence of dCTP and absence of 2 mM Mg2+ Herpes simplex virus
3.5.4.12 0.91
-
dCMP in the presence of dCTP and absence of 2 mM Mg2+ Cricetinae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.12 Mg2+ required for complete activation by dCTP Herpes simplex virus
3.5.4.12 Mg2+ required for complete activation by dCTP Cricetinae
3.5.4.12 Mg2+ required for activation by dCTP and for inhibition by dTTP Herpes simplex virus
3.5.4.12 Mg2+ required for activation by dCTP and for inhibition by dTTP Cricetinae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.12 dCMP + H2O Herpes simplex virus enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis dUMP + NH3
-
?
3.5.4.12 dCMP + H2O Cricetinae enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis dUMP + NH3
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.12 Cricetinae
-
BHK-21/C13 cells, baby-hamster, enzyme from non-infected cells and cells infected by Herpes simplex
-
3.5.4.12 Herpes simplex virus
-
induced by
-
3.5.4.12 Herpes simplex virus
-
host baby-hamster, BHK-21/C13 cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.12 BHK-21/C13 cells , enzyme from non-infected cells and cells infected by Herpes simplex Cricetinae
3.5.4.12 host baby-hamster, BHK-21/C13 cells Herpes simplex virus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.12 BHK-21 cell baby-hamster kidney Cricetinae
-
3.5.4.12 kidney BHK-21/C13 cells, baby-hamster kidney cells Cricetinae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.12 dCMP + H2O the dCMP concentration at half-maximum velocity is lower by enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster than by enzyme from non-infected cells Herpes simplex virus dUMP + NH3
-
?
3.5.4.12 dCMP + H2O the dCMP concentration at half-maximum velocity is lower by enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster than by enzyme from non-infected cells Cricetinae dUMP + NH3
-
?
3.5.4.12 dCMP + H2O enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis Herpes simplex virus dUMP + NH3
-
?
3.5.4.12 dCMP + H2O enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis Cricetinae dUMP + NH3
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.12 37
-
assay at Herpes simplex virus
3.5.4.12 37
-
assay at Cricetinae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.12 additional information
-
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilizes against heat inactivation. Host-cell enzyme is more sensitive to heating than Herpes virus infected enzyme Herpes simplex virus
3.5.4.12 additional information
-
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilizes against heat inactivation. Host-cell enzyme is more sensitive to heating than Herpes virus infected enzyme Cricetinae
3.5.4.12 37
-
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to heating at 37°C than enzyme from non-infected cells. In the presence of 0.125 mM dCTP and 2 mM Mg2+, 100% stable, 20 min at 37°C Herpes simplex virus
3.5.4.12 37
-
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to heating at 37°C than enzyme from non-infected cells. In the presence of 0.125 mM dCTP and 2 mM Mg2+, 100% stable, 20 min at 37°C Cricetinae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.12 additional information
-
enzyme from host-cell and Herpes virus-induced enzyme have a similar pH-optimum Herpes simplex virus
3.5.4.12 additional information
-
enzyme from host-cell and Herpes virus-induced enzyme have a similar pH-optimum Cricetinae