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Literature summary extracted from

  • Chow, K.C.; Xue, H.; Shi, W.; Wong, J.T.F.
    Mutational identification of an essential tryptophan in tryptophanyl-tRNA synthetase of Bacillus subtilis (1992), J. Biol. Chem., 267, 9146-9149.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.2 Trp92 to Phe mutant enzyme Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.2 W92A mutant enzymes Trp92 to Phe, Trp92 to Gln and Trp92 to Ala. Mutant enzymes are inactive in partial reaction of Trp-activation and in overall reaction of tRNA tryptophanylation Bacillus subtilis
6.1.1.2 W92F mutant enzymes Trp92 to Phe, Trp92 to Gln and Trp92 to Ala. Mutant enzymes are inactive in partial reaction of Trp-activation and in overall reaction of tRNA tryptophanylation Bacillus subtilis
6.1.1.2 W92Q mutant enzymes Trp92 to Phe, Trp92 to Gln and Trp92 to Ala. Mutant enzymes are inactive in partial reaction of Trp-activation and in overall reaction of tRNA tryptophanylation Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.2 Bacillus subtilis
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 mutant enzymes Trp92 to Phe, Trp92 to Gln and Trp92 to Ala
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.2 Trp92 to Phe mutant enzyme Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.2 ATP + L-tryptophan + tRNATrp
-
 Bacillus subtilis AMP + diphosphate + L-tryptophan-tRNATrp
-
 ?
6.1.1.2 additional information tryptophan-dependent ATP-diphosphate exchange: tryptophan + ATP + enzyme /Trp-AMP-enzyme + diphosphate Bacillus subtilis ?
-
 ?