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Literature summary extracted from

  • Schumann, J.; Moellering, H.; Jaenicke, R.
    Intrinsic stability and extrinsic stabilization of creatinase from Pseudomonas putida (1993), Biol. Chem. Hoppe-Seyler, 374, 427-434.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.5.3.3 addition of 0.2 M NaCl stabilizes against the solubilizing effect of urea. Methylurea has higher denaturing capacity than urea. At glycerol concentrations up to 20% creatinase is in its native state Pseudomonas putida
3.5.3.3 enzyme shows anomalously low intrinsic stability and is inaccessible to reconstitution after preceding deactivation, denaturation and dissociation Pseudomonas putida
3.5.3.3 upon increasing the concentration of the enzyme from 0.003 to 0.024 mg/ml the apparent concentration of guanidinium chloride where 50% denaturation are observed shifts from 0.35 to 0.44 M Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.3 Pseudomonas putida
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.3.3 enzyme shows anomalously low intrinsic stability and is inaccessible to reconstitution after preceding deactivation, denaturation and dissociation Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.3 creatine + H2O
-
Pseudomonas putida sarcosine + urea
-
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