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Literature summary extracted from

  • Joeres, U.; Kula, M.R.
    Purification and characterisation of a microbial L-carnitine amidase (1994), Appl. Microbiol. Biotechnol., 40, 606-610.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.73 1,10-phenanthroline 10 mM, 18% loss of activity Bacteria
3.5.1.73 5,5'-dithiobis(2-nitrobenzoate) 1 mM, 66% loss of activity Bacteria
3.5.1.73 CoCl2 10 mM, 24% loss of activity Bacteria
3.5.1.73 EDTA 10 mM, 27% loss of activity Bacteria
3.5.1.73 FeCl2 10 mM, 43% loss of activity Bacteria
3.5.1.73 glutathione 10 mM, 28% loss of activity Bacteria
3.5.1.73 HgCl2 1 mM, complete loss of activity Bacteria
3.5.1.73 iodoacetate 1 mM, 28% loss of activity Bacteria
3.5.1.73 KCN 10 mM, 12% loss of activity Bacteria
3.5.1.73 MnCl2 10 mM, 24% loss of activity Bacteria
3.5.1.73 p-hydroxymercuribenzoate 1 mM, 49% loss of activity Bacteria
3.5.1.73 phenylmethanesulfonyl fluoride 1 mM, 77% loss of activity Bacteria
3.5.1.73 ZnCl2 1 mM, 94% loss of activity Bacteria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.73 11.6
-
 L-Carnitine amide
-
 Bacteria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.73 66000
-
 2 * 66000, SDS-PAGE Bacteria
3.5.1.73 125000
-
 gel filtration Bacteria

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.73 Bacteria
-
 DSM 6230, unidentified
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.73
-
 Bacteria

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.73 328
-
-
 Bacteria

Storage Stability

EC Number Storage Stability Organism
3.5.1.73 -20°C, stable for several months Bacteria
3.5.1.73 25°C, in absence of substrate, half-life is 2.6 days Bacteria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.73 L-carnitine amide + H2O
-
 Bacteria L-carnitine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.73 dimer 2 * 66000, SDS-PAGE Bacteria

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.73 40
-
-
 Bacteria

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.73 20 50 about 40% of maximal activity at 20°C and at 50°C Bacteria

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.73 25
-
 in absence of substrate, half-life: 2.6 days Bacteria
3.5.1.73 30
-
 stable up to Bacteria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.73 8.5
-
-
 Bacteria

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.73 6 10 pH 6.0: about 40% of maximal activity, pH 10.0: about 35% of maximal activity Bacteria

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.73 5 9.5 30°C, 30 min, less than 20% loss of activity in the pH-range 5.0-9.5 Bacteria