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Literature summary extracted from

  • Ito, M.; Kurita, T.; Kita, K.
    A novel enzyme that cleaves the N-acyl linkage of ceramides in various glycospingolipids as well as sphingomyelin to produce their lyso forms (1995), J. Biol. Chem., 41, 24370-24374.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.69 Cu2+ 2 mM Pseudomonas sp.
3.5.1.69 EDTA no inhibition Pseudomonas sp.
3.5.1.69 Hg2+ 2 mM Pseudomonas sp.
3.5.1.69 Mn2+ no inhibition Pseudomonas sp.
3.5.1.69 Zn2+ 2 mM Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.69 52000
-
 x * 52000, SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.69 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.69
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
3.5.1.69 -85°C, 2 months, stable Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.69 D-galactosylceramide-3-O-sulfate + H2O
-
 Pseudomonas sp. ?
-
 ?
3.5.1.69 galacosylceramide + H2O
-
 Pseudomonas sp. ?
-
 ?
3.5.1.69 ganglioside asialo-GM1 + H2O
-
 Pseudomonas sp. ?
-
 ?
3.5.1.69 ganglioside GD1a + H2O
-
 Pseudomonas sp. fatty acid + lyso-GD1a-ganglioside
-
 ?
3.5.1.69 ganglioside GM1 + H2O
-
 Pseudomonas sp. octadecanoic acid + lyso-GM1-ganglioside
-
 ?
3.5.1.69 ganglioside GM2 + H2O
-
 Pseudomonas sp. fatty acid + lyso-GM2-ganglioside
-
 ?
3.5.1.69 ganglioside GM3 + H2O
-
 Pseudomonas sp. fatty acid + lyso-GM3-ganglioside
-
 ?
3.5.1.69 ganglioside GQ1b + H2O
-
 Pseudomonas sp. ?
-
 ?
3.5.1.69 globotetraosylceramide + H2O
-
 Pseudomonas sp. ?
-
 ?
3.5.1.69 glucosylceramide + H2O
-
 Pseudomonas sp. ?
-
 ?
3.5.1.69 lactosylceramide + H2O
-
 Pseudomonas sp. ?
-
 ?
3.5.1.69 sphingomyelin + H2O
-
 Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.69 ? x * 52000, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.69 60
-
 30 min, 20% loss of activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.69 5 6
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.69 4 9 stable Pseudomonas sp.