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Literature summary extracted from

  • Hersh, L.B.
    Rat liver omega-amidase. Purofication and properties (1971), Biochemistry, 10, 2884-2891.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.3 5,5'-dithiobis-(2-nitrobenzoic acid) 0.02 mM: complete inhibition; Ellman's reagent Rattus norvegicus
3.5.1.3 ethanol 0.05 M: no inhibition Rattus norvegicus
3.5.1.3 Glutaramate competitive inhibition, 10 mM glutaramate, hydrolysis of methyl alpha-ketoglutarate: 43% inhibition, hydrolysis of ethyl alpha-ketoglutarate: 34% inhibition Rattus norvegicus
3.5.1.3 iodoacetamide 0.2 mM: complete inhibition Rattus norvegicus
3.5.1.3 iodoacetate 0.2 mM: complete inhibition Rattus norvegicus
3.5.1.3 methylamine 0.05 M methylamine: 62% inhibition Rattus norvegicus
3.5.1.3 additional information no inhibition by 0.05 M methanol, ethanol, propanol, ethylamine, propylamine, butylamine, dimethylamine, dierhylamine and triethylamine; no inhibition by EDTA Rattus norvegicus
3.5.1.3 N-ethylmaleimide 0.2 mM: complete inhibition Rattus norvegicus
3.5.1.3 NH4+ 0.05 M ammonia: 72% inhibition Rattus norvegicus
3.5.1.3 p-mercuribenzoate 0.02 mM: complete inhibition Rattus norvegicus
3.5.1.3 succinamate competitive inhibition, 5 mM succinamate, hydrolysis of methyl alpha-ketoglutarate: 57% inhibition, hydrolysis of ethyl alpha-ketoglutarate: 42% inhibition Rattus norvegicus
3.5.1.3 sulfhydryl reagents
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.3 additional information
-
 additional information kinetic studies, criteria and data Rattus norvegicus
3.5.1.3 0.01
-
 2-Oxoglutaramate true Km of omega-amidase for alpha-ketoglutaramate open-chain structure may be of the order of 0.01 mM Rattus norvegicus
3.5.1.3 0.03
-
 gamma-methyl 2-oxoglutarate hydrolysis Rattus norvegicus
3.5.1.3 0.06
-
 gamma-ethyl 2-oxoglutarate hydrolysis Rattus norvegicus
3.5.1.3 0.2
-
 succinamate hydrolysis Rattus norvegicus
3.5.1.3 0.7
-
 methyl succinate hydrolysis Rattus norvegicus
3.5.1.3 0.8
-
 succinyl hydroxamate hydrolysis Rattus norvegicus
3.5.1.3 1.8
-
 methyl glutarate hydrolysis Rattus norvegicus
3.5.1.3 3.1
-
 Glutaramate hydrolysis Rattus norvegicus
3.5.1.3 3.3
-
 2-oxoglutarate hydrolysis Rattus norvegicus
3.5.1.3 3.6
-
 hydroxylamine hydroxaminolysis, substrate: methyl glutarate Rattus norvegicus
3.5.1.3 4
-
 hydroxylamine hydroxaminolysis, substrate: glutaramate Rattus norvegicus
3.5.1.3 5.4
-
 hydroxylamine hydroxaminolysis, substrate: succinamate Rattus norvegicus
3.5.1.3 6.8
-
 propyl glutarate hydrolysis Rattus norvegicus
3.5.1.3 8
-
 methyl succinate hydroxaminolysis Rattus norvegicus
3.5.1.3 8.3
-
 ethyl succinate hydrolysis Rattus norvegicus
3.5.1.3 9.9
-
 ethyl glutarate hydrolysis Rattus norvegicus
3.5.1.3 17
-
 succinamate hydroxaminolysis Rattus norvegicus
3.5.1.3 17
-
 ethyl succinate hydroxaminolysis Rattus norvegicus
3.5.1.3 18
-
 propyl glutarate hydroxaminolysis Rattus norvegicus
3.5.1.3 25
-
 Glutaramate hydroxaminolysis Rattus norvegicus
3.5.1.3 31
-
 ethyl glutarate hydroxaminolysis Rattus norvegicus
3.5.1.3 52
-
 methyl glutarate hydroxaminolysis Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.3 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.3 additional information no effect of 0.05 M NaCl, KCl, MgCl2, CaCl2, or BaCl2 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.3 58000
-
 liver, high-speed sedimentation equilibrium and amino-acid analysis Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.3 2-oxoglutaramate + H2O Rattus norvegicus alpha-ketoglutaramate 2-oxoglutarate + NH3
-
 ?
3.5.1.3 additional information Rattus norvegicus conversion of glutamine into alpha-ketoglutarate and ammonia is brought about by two distinct enzymes: glutamine transaminase and omega-amidase ?
-
 ?
3.5.1.3 additional information Rattus norvegicus enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine ?
-
 ?
3.5.1.3 additional information Rattus norvegicus second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.3
-
 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.3 a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3 mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.3 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.3 additional information
-
-
 Rattus norvegicus
3.5.1.3 11.7
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.5.1.3 3°C, 1 mM dithiothreitol, 2 weeks, less than 10% activity lost Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.3 2-oxoglutaramate + H2O alpha-ketoglutaramate Rattus norvegicus 2-oxoglutarate + NH3
-
 ?
3.5.1.3 2-oxoglutaramate + hydroxylamine no hydroxaminolysis of alpha-ketoglutaramate at pH 8 Rattus norvegicus 2-oxoglutaryl hydroxamate + NH3
-
 ?
3.5.1.3 2-oxoglutaramate + methylamine alpha-ketoglutaramate Rattus norvegicus ?
-
 ?
3.5.1.3 2-oxoglutaramate + methylamine transamidation Rattus norvegicus ?
-
 ?
3.5.1.3 2-oxosuccinamate + H2O alpha-ketosuccinamate Rattus norvegicus 2-oxosuccinate + NH3
-
 ?
3.5.1.3 butyl glutarate + hydoxylamine hydroxaminolysis Rattus norvegicus glutaramate + butanol
-
 ?
3.5.1.3 delta-ethyl 2-oxoglutarate + H2O delta-ethyl alpha-ketoglutarate Rattus norvegicus ?
-
 ?
3.5.1.3 delta-methyl 2-oxoglutarate + H2O delta-methyl alpha-ketoglutarate Rattus norvegicus ?
-
 ?
3.5.1.3 ethyl glutarate + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.1.3 ethyl glutarate + hydoxylamine hydroxaminolysis Rattus norvegicus glutaramate + ethanol
-
 ?
3.5.1.3 ethyl glutarate + methylamine transamidation Rattus norvegicus ?
-
 ?
3.5.1.3 ethyl succinate + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.1.3 ethyl succinate + hydoxylamine hydroxaminolysis Rattus norvegicus succinamate + ethanol
-
 ?
3.5.1.3 ethyl succinate + methylamine transamidation Rattus norvegicus ?
-
 ?
3.5.1.3 gamma-ethyl 2-oxoglutarate + H2O gamma-methyl alpha-ketoglutarate Rattus norvegicus ?
-
 ?
3.5.1.3 gamma-ethyl 2-oxoglutarate + methylamine transamidation, poor Rattus norvegicus ?
-
 ?
3.5.1.3 gamma-ethyl 2-oxoglutarate + methylamine gamma-ethyl alpha-ketoglutarate Rattus norvegicus ?
-
 ?
3.5.1.3 gamma-methyl 2-oxoglutarate + H2O gamma-methyl alpha-ketoglutarate Rattus norvegicus ?
-
 ?
3.5.1.3 gamma-methyl 2-oxoglutarate + methylamine transamidation, very poor Rattus norvegicus ?
-
 ?
3.5.1.3 glutaramate + H2O
-
 Rattus norvegicus glutarate + NH3
-
 ?
3.5.1.3 glutaramate + hydroxylamine hydroxaminolysis Rattus norvegicus glutaryl hydroxamate + NH3
-
 ?
3.5.1.3 glutaramate + methylamine transamidation Rattus norvegicus ?
-
 ?
3.5.1.3 glutarate + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.1.3 methyl glutarate + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.1.3 methyl glutarate + hydoxylamine hydroxaminolysis Rattus norvegicus glutaramate + methanol
-
 ?
3.5.1.3 methyl glutarate + methylamine transamidation Rattus norvegicus ?
-
 ?
3.5.1.3 methyl succinate + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.1.3 methyl succinate + hydoxylamine hydroxaminolysis Rattus norvegicus succinamate + methanol
-
 ?
3.5.1.3 methyl succinate + methylamine transamidation Rattus norvegicus ?
-
 ?
3.5.1.3 additional information substrate specificity Rattus norvegicus ?
-
 ?
3.5.1.3 additional information broad specificity Rattus norvegicus ?
-
 ?
3.5.1.3 additional information no hydrolysis of glutamine, asparagine, N-methyl-alpha-ketoglutaramate, dimethyl glutarate, or the anilides of succinate or glutarate. No hydrolysis of N-methylsuccinamate or N-ethylsuccinamate Rattus norvegicus ?
-
 ?
3.5.1.3 additional information enzyme has esterolytic activity Rattus norvegicus ?
-
 ?
3.5.1.3 additional information hydrolyzes monomethyl and monoethyl esters of alpha-ketoglutarate, gamma-ester, glutarate, succinate, and p-chloro, p-methyl, and unsubstituted phenyl esters of glutarate Rattus norvegicus ?
-
 ?
3.5.1.3 additional information hydrolyzes monomethyl and ethyl esters of alpha-ketoglutarate, delta esters, succinate, and glutarate Rattus norvegicus ?
-
 ?
3.5.1.3 additional information catalyzes methylamide formation Rattus norvegicus ?
-
 ?
3.5.1.3 additional information no hydroxaminolysis of delta-methyl glutamate, delta-ethyl glutamate, malonate, glutamate, glutarate, aspartate, succinate, glutamine, asparagine, succinanilate, glutaranilate, alpha-ketoglutaramate N-methylsuccinamate, and N-ethylsuccinamate at pH 8 Rattus norvegicus ?
-
 ?
3.5.1.3 additional information hydrolyzes monomethyl and monoethyl esters of alpha-ketoglutarate, delta-ester, glutarate, and succinate, and monopropyl ester of glutarate Rattus norvegicus ?
-
 ?
3.5.1.3 additional information catalyzes hydroxaminolysis and transamidation reactions with esters and amides Rattus norvegicus ?
-
 ?
3.5.1.3 additional information conversion of glutamine into alpha-ketoglutarate and ammonia is brought about by two distinct enzymes: glutamine transaminase and omega-amidase Rattus norvegicus ?
-
 ?
3.5.1.3 additional information enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine Rattus norvegicus ?
-
 ?
3.5.1.3 additional information second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway Rattus norvegicus ?
-
 ?
3.5.1.3 propyl glutarate + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.1.3 propyl glutarate + hydoxylamine hydroxaminolysis Rattus norvegicus glutaramate + propanol
-
 ?
3.5.1.3 succinamate + H2O
-
 Rattus norvegicus succinic acid + NH3
-
 ?
3.5.1.3 succinamate + hydroxylamine hydroxaminolysis Rattus norvegicus succinyl hydroxamate + NH3
-
 r
3.5.1.3 succinamate + methylamine transamidation Rattus norvegicus ?
-
 ?
3.5.1.3 succinate + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.1.3 succinyl hydroxamate + H2O
-
 Rattus norvegicus succinate + hydroxylamine
-
 r

Subunits

EC Number Subunits Comment Organism
3.5.1.3 dimer liver, 2 * 27000-28000, two subunits may be nonidentical, high-speed sedimentation equilibrium in the presence of 7 M guanidine hydrochloride or 7 M urea containing 0.1 M 2-mercaptoethanol Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.3 30
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.3 50
-
 30 min, 0.02 M Tris-HCl, pH 8.5, no loss of activity Rattus norvegicus
3.5.1.3 60
-
 5 min, 70% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.3 additional information
-
-
 Rattus norvegicus
3.5.1.3 8.5
-
 assay at Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.3 additional information
-
-
 Rattus norvegicus
3.5.1.3 4
-
 20 min, 90% loss of activity Rattus norvegicus
3.5.1.3 5
-
 20 min, 32% loss of activity Rattus norvegicus
3.5.1.3 6 10 20 min, 30°C, stable between, no irreversible inactivation Rattus norvegicus