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Literature summary extracted from

  • Wang, H.J.; Bai, J.H.; Liu, D.S.; Zhang, T.; Zhou, H.M.
    Preparation and properties of immobilized pig kidney aminoacylase and optical resolution of N-acyl-DL-alanine (1999), Appl. Biochem. Biotechnol., 76, 183-191.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.14 synthesis
-
Sus scrofa
3.5.1.14 synthesis
-
Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.14 Co2+
-
Aspergillus oryzae
3.5.1.14 Co2+ activates free and immobilized enzyme Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.14 N-acetyl-L-methionine Sus scrofa
-
L-methionine + acetate
-
?
3.5.1.14 N-acetyl-L-methionine Aspergillus oryzae
-
L-methionine + acetate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Aspergillus oryzae
-
-
-
3.5.1.14 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.14 kidney
-
Sus scrofa
-

Storage Stability

EC Number Storage Stability Organism
3.5.1.14 4°C, immobilized enzyme stable for more than 2 months without significant loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 N-acetyl-DL-methionine + H2O
-
Sus scrofa DL-methionine + acetate
-
?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
Aspergillus oryzae DL-methionine + acetate
-
?
3.5.1.14 N-acetyl-L-alanine + H2O
-
Sus scrofa L-alanine + acetate
-
?
3.5.1.14 N-acetyl-L-alanine + H2O
-
Aspergillus oryzae L-alanine + acetate
-
?
3.5.1.14 N-acetyl-L-methionine
-
Sus scrofa L-methionine + acetate
-
?
3.5.1.14 N-acetyl-L-methionine
-
Aspergillus oryzae L-methionine + acetate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.14 65
-
immobilized enzyme Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.14 70
-
immobilized enzyme more thermally stable, preserved during prolonged incubation at 70°C, soluble enzyme completely inactivated in 10 min under same conditions Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.14 7.5
-
immobilized enzyme Sus scrofa
3.5.1.14 8
-
-
Sus scrofa