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Literature summary extracted from

  • Wakayama, M.; Shiiba, E.; Sakai, K.; Moriguchi, M.
    Purification and characterization of L-aminoacylase from Pseudomonas maltophila B1 (1998), J. Ferment. Bioeng., 85, 278-282.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.1.14 synthesis
-
 Sus scrofa
3.5.1.14 synthesis
-
 Aspergillus oryzae
3.5.1.14 synthesis thermostable enzyme useful for industrial production, optical resolution of DL-amino acids on an industrial scale, utilization in industrial production of stereoisomers from racemates Bacillus sp. (in: Bacteria)
3.5.1.14 synthesis thermostable enzyme useful for industrial production, optical resolution of DL-amino acids on an industrial scale, utilization in industrial production of stereoisomers from racemates Stenotrophomonas maltophilia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.14 1,10-phenanthroline
-
 Stenotrophomonas maltophilia
3.5.1.14 1,10-phenanthroline
-
 Sus scrofa
3.5.1.14 2,3-Butanedione
-
 Stenotrophomonas maltophilia
3.5.1.14 diethylpyrocarbonate
-
 Stenotrophomonas maltophilia
3.5.1.14 DL-lactate
-
 Aspergillus oryzae
3.5.1.14 DL-lactate
-
 Stenotrophomonas maltophilia
3.5.1.14 DL-lactate
-
 Sus scrofa
3.5.1.14 glyoxylate
-
 Stenotrophomonas maltophilia
3.5.1.14 phenylboronic acid
-
 Stenotrophomonas maltophilia
3.5.1.14 Phenylglyoxal
-
 Stenotrophomonas maltophilia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.14 2.32
-
 N-acetyl-L-alanine
-
 Stenotrophomonas maltophilia
3.5.1.14 12.7
-
 N-acetyl-DL-aspartate
-
 Stenotrophomonas maltophilia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.14 Ca2+
-
 Stenotrophomonas maltophilia
3.5.1.14 Co2+ enzyme strongly activated by Co2+ ions Sus scrofa
3.5.1.14 Co2+ enzyme strongly activated by Co2+ ions Bacillus sp. (in: Bacteria)
3.5.1.14 Co2+ enzyme strongly activated by Co2+ ions Aspergillus oryzae
3.5.1.14 Co2+ enzyme strongly activated by Co2+ ions Stenotrophomonas maltophilia
3.5.1.14 Zn2+ enzyme contains Zn as prosthetic metal Sus scrofa
3.5.1.14 Zn2+ enzyme contains Zn as prosthetic metal Bacillus sp. (in: Bacteria)
3.5.1.14 Zn2+ enzyme contains Zn as prosthetic metal Aspergillus oryzae
3.5.1.14 Zn2+ enzyme contains Zn as prosthetic metal Stenotrophomonas maltophilia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.14 45000
-
 2 * 45000, denaturated intestinal mucosa enzyme, SDS-PAGE Sus scrofa
3.5.1.14 50000
-
 2 * 50000, native enzyme SDS-PAGE Stenotrophomonas maltophilia
3.5.1.14 108000
-
 gel filtration, native enzyme Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.14 N-acetyl-L-methionine Sus scrofa
-
 L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine Bacillus sp. (in: Bacteria)
-
 L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine Aspergillus oryzae
-
 L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine Stenotrophomonas maltophilia
-
 L-methionine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Aspergillus oryzae
-
-
-
3.5.1.14 Bacillus sp. (in: Bacteria)
-
-
-
3.5.1.14 Stenotrophomonas maltophilia
-
 gram-negative, motile, nonsporeforming rod-shaped bacterium, B1
-
3.5.1.14 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.14
-
 Stenotrophomonas maltophilia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 N-acetyl-DL-aspartate + H2O
-
 Bacillus sp. (in: Bacteria) acetate + DL-aspartate
-
 ?
3.5.1.14 N-acetyl-DL-aspartate + H2O
-
 Stenotrophomonas maltophilia acetate + DL-aspartate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Sus scrofa DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Bacillus sp. (in: Bacteria) DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Aspergillus oryzae DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Stenotrophomonas maltophilia DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-DL-serine + H2O
-
 Stenotrophomonas maltophilia acetate + DL-serine
-
 ?
3.5.1.14 N-acetyl-glycine + H2O
-
 Bacillus sp. (in: Bacteria) acetate + glycine
-
 ?
3.5.1.14 N-acetyl-glycine + H2O
-
 Aspergillus oryzae acetate + glycine
-
 ?
3.5.1.14 N-acetyl-glycine + H2O
-
 Stenotrophomonas maltophilia acetate + glycine
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Sus scrofa L-alanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Bacillus sp. (in: Bacteria) L-alanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Aspergillus oryzae L-alanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Stenotrophomonas maltophilia L-alanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-glutamine + H2O
-
 Sus scrofa L-glutamine + acetate
-
 ?
3.5.1.14 N-acetyl-L-glutamine + H2O
-
 Bacillus sp. (in: Bacteria) L-glutamine + acetate
-
 ?
3.5.1.14 N-acetyl-L-glutamine + H2O
-
 Aspergillus oryzae L-glutamine + acetate
-
 ?
3.5.1.14 N-acetyl-L-glutamine + H2O
-
 Stenotrophomonas maltophilia L-glutamine + acetate
-
 ?
3.5.1.14 N-acetyl-L-leucine + H2O
-
 Sus scrofa acetate + L-leucine
-
 ?
3.5.1.14 N-acetyl-L-leucine + H2O
-
 Bacillus sp. (in: Bacteria) acetate + L-leucine
-
 ?
3.5.1.14 N-acetyl-L-leucine + H2O
-
 Stenotrophomonas maltophilia acetate + L-leucine
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Sus scrofa L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Bacillus sp. (in: Bacteria) L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Aspergillus oryzae L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Stenotrophomonas maltophilia L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-phenylalanine + H2O
-
 Sus scrofa L-phenylalanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-phenylalanine + H2O
-
 Bacillus sp. (in: Bacteria) L-phenylalanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-phenylalanine + H2O
-
 Stenotrophomonas maltophilia L-phenylalanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-valine + H2O
-
 Sus scrofa L-valine + acetate
-
 ?
3.5.1.14 N-acetyl-L-valine + H2O
-
 Bacillus sp. (in: Bacteria) L-valine + acetate
-
 ?
3.5.1.14 N-acetyl-L-valine + H2O
-
 Stenotrophomonas maltophilia L-valine + acetate
-
 ?
3.5.1.14 N-chloroacetyl-DL-phenylalanine + H2O
-
 Sus scrofa chloroacetate + phenylalanine
-
 ?
3.5.1.14 N-chloroacetyl-DL-phenylalanine + H2O
-
 Bacillus sp. (in: Bacteria) chloroacetate + phenylalanine
-
 ?
3.5.1.14 N-chloroacetyl-DL-phenylalanine + H2O
-
 Aspergillus oryzae chloroacetate + phenylalanine
-
 ?
3.5.1.14 N-chloroacetyl-DL-phenylalanine + H2O
-
 Stenotrophomonas maltophilia chloroacetate + phenylalanine
-
 ?
3.5.1.14 N-chloroacetyl-DL-valine + H2O
-
 Sus scrofa chloroacetate + DL-valine
-
 ?
3.5.1.14 N-chloroacetyl-DL-valine + H2O
-
 Bacillus sp. (in: Bacteria) chloroacetate + DL-valine
-
 ?
3.5.1.14 N-chloroacetyl-DL-valine + H2O
-
 Stenotrophomonas maltophilia chloroacetate + DL-valine
-
 ?
3.5.1.14 N-formyl-L-methionine + H2O
-
 Stenotrophomonas maltophilia formate + L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.14 dimer
-
 Aspergillus oryzae
3.5.1.14 dimer 2 * 45000, denaturated intestinal mucosa enzyme, SDS-PAGE Sus scrofa
3.5.1.14 dimer 2 * 50000, native enzyme SDS-PAGE Stenotrophomonas maltophilia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.14 55
-
-
 Stenotrophomonas maltophilia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.14 8
-
-
 Sus scrofa
3.5.1.14 8
-
-
 Stenotrophomonas maltophilia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.14 6 10
-
 Stenotrophomonas maltophilia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.14 5 8 stable in wide pH-range, 5-8 at 30°C and 7-7.5 at 55°C Stenotrophomonas maltophilia