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Literature summary extracted from

  • Sakanyan, V.; Desmarez, L.; Legrain, C.; Charlier, D.; Mett, I.; Kochikyan, A.; Savchenko, A.; Boyen, A.; Falmagne, P.; Pierard, A.; Glansdorff, N.
    Gene cloning, sequence analysis, purification, and characterization of an thermostable aminoacylase from Bacillus stearothermophilus (1993), Appl. Environ. Microbiol., 59, 3878-3888.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.14 synthesis
-
 Sus scrofa
3.5.1.14 synthesis
-
 Aspergillus oryzae
3.5.1.14 synthesis thermostable enzyme useful for industrial production, optical resolution of DL-amino acids on an industrial scale, utilization in industrial production of stereoisomers from racemates Bacillus sp. (in: Bacteria)

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.14 genomic DNA fragment encoding aminoacylase cloned, sequenced and expressed in Escherichia coli Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.14 Co2+ enzyme strongly activated by Co2+ ions Sus scrofa
3.5.1.14 Co2+ enzyme strongly activated by Co2+ ions Bacillus sp. (in: Bacteria)
3.5.1.14 Co2+ enzyme strongly activated by Co2+ ions Aspergillus oryzae
3.5.1.14 Mn2+
-
 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.14 42000
-
 4 * 42000, SDS-PAGE Bacillus sp. (in: Bacteria)
3.5.1.14 168000
-
 gel filtration, low glutaraldehyde concentration, native tetrameric form Bacillus sp. (in: Bacteria)
3.5.1.14 180000
-
 gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.14 N-acetyl-L-methionine Homo sapiens
-
 L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine Sus scrofa
-
 L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine Bacillus sp. (in: Bacteria)
-
 L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine Aspergillus oryzae
-
 L-methionine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Aspergillus oryzae
-
-
-
3.5.1.14 Bacillus sp. (in: Bacteria)
-
 stearothermophilus
-
3.5.1.14 Homo sapiens
-
 human
-
3.5.1.14 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.14 recombinant protein, expressed in Escherichia coli Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 benzoyl-DL-phenylalanine + H2O
-
 Bacillus sp. (in: Bacteria) benzoate + DL-phenylalanine
-
 ?
3.5.1.14 glycyl-L-methionine + H2O
-
 Bacillus sp. (in: Bacteria) glycine + L-methionine
-
 ?
3.5.1.14 glycyl-L-tyrosine + H2O
-
 Bacillus sp. (in: Bacteria) glycine + L-tyrosine
-
 ?
3.5.1.14 L-methionylglycine + H2O
-
 Bacillus sp. (in: Bacteria) glycine + L-methionine
-
 ?
3.5.1.14 N-acetyl-DL-aspartate + H2O
-
 Bacillus sp. (in: Bacteria) acetate + DL-aspartate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Homo sapiens DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Sus scrofa DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Bacillus sp. (in: Bacteria) DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Aspergillus oryzae DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-glycine + H2O
-
 Bacillus sp. (in: Bacteria) acetate + glycine
-
 ?
3.5.1.14 N-acetyl-glycine + H2O
-
 Aspergillus oryzae acetate + glycine
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Sus scrofa L-alanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Bacillus sp. (in: Bacteria) L-alanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Aspergillus oryzae L-alanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-arginine + H2O
-
 Sus scrofa acetate + L-arginine
-
 ?
3.5.1.14 N-acetyl-L-glutamine + H2O
-
 Sus scrofa L-glutamine + acetate
-
 ?
3.5.1.14 N-acetyl-L-glutamine + H2O
-
 Bacillus sp. (in: Bacteria) L-glutamine + acetate
-
 ?
3.5.1.14 N-acetyl-L-glutamine + H2O
-
 Aspergillus oryzae L-glutamine + acetate
-
 ?
3.5.1.14 N-acetyl-L-histidine + H2O
-
 Sus scrofa acetate + L-histidine
-
 ?
3.5.1.14 N-acetyl-L-leucine + H2O
-
 Sus scrofa acetate + L-leucine
-
 ?
3.5.1.14 N-acetyl-L-leucine + H2O
-
 Bacillus sp. (in: Bacteria) acetate + L-leucine
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Homo sapiens L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Sus scrofa L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Bacillus sp. (in: Bacteria) L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Aspergillus oryzae L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-ornithine + H2O
-
 Bacillus sp. (in: Bacteria) acetate + L-ornithine
-
 ?
3.5.1.14 N-acetyl-L-phenylalanine + H2O
-
 Sus scrofa L-phenylalanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-phenylalanine + H2O
-
 Bacillus sp. (in: Bacteria) L-phenylalanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-tryptophan + H2O
-
 Sus scrofa acetate + L-tryptophan
-
 ?
3.5.1.14 N-acetyl-L-tyrosine + H2O
-
 Bacillus sp. (in: Bacteria) tyrosine + acetate
-
 ?
3.5.1.14 N-acetyl-L-valine + H2O
-
 Sus scrofa L-valine + acetate
-
 ?
3.5.1.14 N-acetyl-L-valine + H2O
-
 Bacillus sp. (in: Bacteria) L-valine + acetate
-
 ?
3.5.1.14 N-chloroacetyl-DL-phenylalanine + H2O
-
 Sus scrofa chloroacetate + phenylalanine
-
 ?
3.5.1.14 N-chloroacetyl-DL-phenylalanine + H2O
-
 Bacillus sp. (in: Bacteria) chloroacetate + phenylalanine
-
 ?
3.5.1.14 N-chloroacetyl-DL-phenylalanine + H2O
-
 Aspergillus oryzae chloroacetate + phenylalanine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.14 tetramer 4 * 42000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.14 70
-
-
 Bacillus sp. (in: Bacteria)