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Literature summary extracted from

  • Szajani, B.; Ivony, K.; Boross, L.
    Comparative studies on soluble and immobilized pig kidney aminoacylase (1980), J. Appl. Biochem., 2, 72-80.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.1.14 synthesis industrial applications Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.14 1.4
-
 N-acetyl-L-methionine immobilized enzyme Sus scrofa
3.5.1.14 6
-
 N-acetyl-L-methionine soluble enzyme Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.14 N-acetyl-L-methionine Sus scrofa
-
 L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine Aspergillus oryzae
-
 L-methionine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Aspergillus oryzae
-
-
-
3.5.1.14 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.14
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.14 kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Sus scrofa DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Aspergillus oryzae DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Sus scrofa L-alanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Aspergillus oryzae L-alanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Sus scrofa L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Aspergillus oryzae L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-valine + H2O
-
 Sus scrofa L-valine + acetate
-
 ?
3.5.1.14 N-acetyl-O-benzyl-DL-serine + H2O no hydrolysis with immobilized enzyme Sus scrofa acetate + O-benzyl-DL-serine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.14 dimer dissociable enzyme, monomer and dimer forms Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.14 60
-
 soluble enzyme Sus scrofa
3.5.1.14 65
-
 immobilized enzyme Sus scrofa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.14 20 80
-
 Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.14 70
-
 immobilized enzyme more thermally stable, preserved during prolonged incubation at 70°C, soluble enzyme completely inactivated in 10 min under same conditions Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.14 7
-
-
 Aspergillus oryzae
3.5.1.14 7
-
 soluble enzyme Sus scrofa
3.5.1.14 7.3 7.6 immobilized enzyme Sus scrofa
3.5.1.14 7.5
-
 immobilized enzyme Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.14 5.5 8.5 stable in 0.1 M potassium phosphate buffer at 55, 60 and 70°C, maximum stability from pH 6.5-6.6, immobilized enzyme at pH 7.2 Sus scrofa