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Literature summary extracted from

  • Matsumoto, J.; Nagai, S.
    Amidohydrolases for N-short and long chain fatty acyl-L-amino acids from Mycobacteria (1972), J. Biochem., 72, 269-279.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.14 dithioerythritol with palmitoylaspartate as substrate Mycobacterium sp.
3.5.1.14 dithiothreitol slight activation of short acyl aminoacylase at 0.2 mM Mycobacterium sp.
3.5.1.14 mercaptoethanol
-
 Mycobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.14 Co2+ inhibitory at concentration 1 mM Mycobacterium sp.
3.5.1.14 Cu2+
-
 Mycobacterium sp.
3.5.1.14 Hg2+
-
 Mycobacterium sp.
3.5.1.14 iodoacetic acid
-
 Mycobacterium sp.
3.5.1.14 Mn2+ inhibitory at concentration 1 mM Mycobacterium sp.
3.5.1.14 p-chloromercuribenzoate
-
 Mycobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.14 0.15
-
 palmitoylaspartate
-
 Mycobacterium sp.
3.5.1.14 2.5
-
 N-acetyl-L-valine
-
 Mycobacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.14 Ca2+
-
 Mycobacterium sp.
3.5.1.14 Co2+ enzyme strongly activated by Co2+ ions Mycobacterium sp.
3.5.1.14 Co2+ inhibitory at concentration 1 mM Mycobacterium sp.
3.5.1.14 Mg2+
-
 Mycobacterium sp.
3.5.1.14 Mn2+ inhibitory at concentration 1 mM Mycobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.14 40000
-
 gel filtration, long acyl aminoacylase, hydrolysis of N-long chain fatty acyl amino acids Mycobacterium sp.
3.5.1.14 88000
-
 gel filtration, short acyl aminoacylase Mycobacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.14 N-acetyl-L-methionine Mycobacterium sp.
-
 L-methionine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Mycobacterium sp.
-
 avium, smegmatis, phlei
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.14 both short acyl aminoacylase and long acyl aminoacylase Mycobacterium sp.

Storage Stability

EC Number Storage Stability Organism
3.5.1.14 -20°C, 0.2 mM DTT buffer, pH 6.8, retains its acivity for 6 months Mycobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 N-acetyl-DL-aspartate + H2O
-
 Mycobacterium sp. acetate + DL-aspartate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Mycobacterium sp. DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-glutamine + H2O
-
 Mycobacterium sp. L-glutamine + acetate
-
 ?
3.5.1.14 N-acetyl-L-leucine + H2O
-
 Mycobacterium sp. acetate + L-leucine
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Mycobacterium sp. L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-valine + H2O
-
 Mycobacterium sp. L-valine + acetate
-
 ?
3.5.1.14 palmitoyl-L-aspartate + H2O
-
 Mycobacterium sp. palmitate + L-aspartate
-
 ?
3.5.1.14 palmitoyl-L-phenylalanine + H2O
-
 Mycobacterium sp. palmitate + L-phenylalanine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.14 5.7
-
 with palmitoylaspartate as substrate Mycobacterium sp.