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Literature summary extracted from

  • Shallom, D.; Belakhov, V.; Solomon, D.; Gilead-Gropper, S.; Baasov, T.; Shoham, G.; Shoham, Y.
    The identification of the acid-base catalyst of alpha-arabinofuranosidase from Geobacillus stearothermophilus T-6, a family 51 glycoside hydrolase (2002), FEBS Lett., 514, 163-167.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.55 agriculture bioconversion of lignocellulose material for the improvement of animal feedstock digestibility Geobacillus stearothermophilus
3.2.1.55 nutrition
-
 Bacillus subtilis
3.2.1.55 nutrition aromatic potential in wine, exploitation in flavor improvement and wine aromatization Geobacillus stearothermophilus
3.2.1.55 paper production
-
 Bacillus subtilis
3.2.1.55 paper production potential industrial use in biobleaching of paper pulp, bioconversion of lignocellulose material to fermentative products and for the improvement of animal feedstock digestibility Geobacillus stearothermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 cloning of the abfA T-6 gene, PCR, T7 polymerase expression vector pET9d, expressed in Escherichia coli BL21(DE3) Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.1
-
 2,5-dinitrophenyl beta-D-xylopyranoside mutant E175A Geobacillus stearothermophilus
3.2.1.55 0.9
-
 3,4-dinitrophenyl beta-D-xylopyranoside mutant E175A Geobacillus stearothermophilus
3.2.1.55 4.4
-
 3,4-dinitrophenyl beta-D-xylopyranoside wild-type Abfa T-6 Geobacillus stearothermophilus
3.2.1.55 5.3
-
 2-nitrophenyl beta-D-xylopyranoside mutant E175A Geobacillus stearothermophilus
3.2.1.55 8
-
 2,5-dinitrophenyl beta-D-xylopyranoside wild-type Abfa T-6 Geobacillus stearothermophilus
3.2.1.55 15.3
-
 2-nitrophenyl beta-D-xylopyranoside wild-type Abfa T-6 Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Agrobacterium tumefaciens
-
-
-
3.2.1.55 Bacillus subtilis
-
-
-
3.2.1.55 Geobacillus stearothermophilus
-
 T-6
-
3.2.1.55 Geobacillus stearothermophilus
-
 mutant E175A
-
3.2.1.55 Geobacillus stearothermophilus T-6
-
 T-6
-
3.2.1.55 Halalkalibacterium halodurans
-
-
-
3.2.1.55 Mesorhizobium loti
-
-
-
3.2.1.55 Sinorhizobium meliloti
-
-
-
3.2.1.55 Streptomyces coelicolor
-
-
-
3.2.1.55 Streptomyces lividans
-
 66strain 1326
-
3.2.1.55 Streptomyces lividans 66 1326
-
 66strain 1326
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 recombinant enzyme, expressed in Escherichia coli BL21(DE3) Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 2,5-dinitrophenyl beta-D-xylopyranoside + H2O
-
 Geobacillus stearothermophilus 2,5-dinitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.55 2,5-dinitrophenyl beta-D-xylopyranoside + H2O
-
 Geobacillus stearothermophilus T-6 2,5-dinitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.55 2-nitrophenyl beta-D-xylopyranoside + H2O
-
 Geobacillus stearothermophilus 2-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.55 2-nitrophenyl beta-D-xylopyranoside + H2O
-
 Geobacillus stearothermophilus T-6 2-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.55 3,4-dinitrophenyl beta-D-xylopyranoside + H2O
-
 Geobacillus stearothermophilus 3,4-dinitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.55 3,4-dinitrophenyl beta-D-xylopyranoside + H2O
-
 Geobacillus stearothermophilus T-6 3,4-dinitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans 66 1326 p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus T-6 p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Sinorhizobium meliloti L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces coelicolor L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Agrobacterium tumefaciens L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Mesorhizobium loti L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Halalkalibacterium halodurans L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans 66 1326 L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus T-6 L-arabinose + phenol
-
 ir