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Literature summary extracted from

  • Tsujibo, H.; Takada, C.; Wakamatsu, Y.; Kosaka, M.; Tsuji, A.; Miyamoto, K.; Inamori, Y.
    Cloning and expression of an alpha-L-arabinofuranosidase gene (stxIV) from Streptomyces thermoviolaceus OPC-520 (2002), Biosci. Biotechnol. Biochem., 66, 434-438.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 abfB gene, nucleotide sequence determined Aspergillus nidulans
3.2.1.55 alpha-AF cDNA cloned from Trichoderma reesei RUT C-30 chromosomal DNA using PCR technique, trying to express this gene in the yeast Schizosaccharomyces pombe, expression in Saccharomyces cerevisiae Trichoderma reesei
3.2.1.55 cDNA clones isolated Hordeum vulgare
3.2.1.55 cloning and sequencing of the gene stxIV encoding alpha-L-arabinofuranosidase, expressed in Escherichia coli JM109 Streptomyces thermoviolaceus
3.2.1.55 gene cloning of 2 isoenzymes, PCR products subcloned into pCR2.1 plasmids Streptomyces chartreusis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Cd2+
-
 Streptomyces thermoviolaceus
3.2.1.55 Co2+
-
 Streptomyces thermoviolaceus
3.2.1.55 Cu2+
-
 Streptomyces thermoviolaceus
3.2.1.55 Cu2+
-
 Thermoclostridium stercorarium
3.2.1.55 EDTA
-
 Aspergillus sojae
3.2.1.55 EDTA
-
 Streptomyces thermoviolaceus
3.2.1.55 Hg2+
-
 Aspergillus nidulans
3.2.1.55 Hg2+
-
 Streptomyces thermoviolaceus
3.2.1.55 Hg2+
-
 Thermoclostridium stercorarium
3.2.1.55 Ni2+
-
 Streptomyces thermoviolaceus
3.2.1.55 p-chloromercuribenzoate
-
 Streptomyces thermoviolaceus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.55 Ca2+ weak stimulation of the enzyme activity Streptomyces thermoviolaceus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 35990
-
 calculated from amino acid sequence Streptomyces thermoviolaceus
3.2.1.55 37000
-
-
 Streptomyces chartreusis
3.2.1.55 37000
-
 SDS-PAGE Streptomyces thermoviolaceus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 oat spelt xylan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Aspergillus sojae
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Trichoderma reesei
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Aspergillus awamori
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Thermoclostridium stercorarium
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Streptomyces thermoviolaceus
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Streptomyces thermoviolaceus OPC-520
-
 L-arabinose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aspergillus awamori
-
-
-
3.2.1.55 Aspergillus nidulans
-
-
-
3.2.1.55 Aspergillus niger
-
-
-
3.2.1.55 Aspergillus sojae
-
-
-
3.2.1.55 Hordeum vulgare
-
-
-
3.2.1.55 Streptomyces chartreusis
-
 GS901
-
3.2.1.55 Streptomyces chartreusis GS901
-
 GS901
-
3.2.1.55 Streptomyces chattanoogensis
-
-
-
3.2.1.55 Streptomyces coelicolor O54161
-
-
3.2.1.55 Streptomyces lividans
-
 66strain 1326
-
3.2.1.55 Streptomyces lividans 66 1326
-
 66strain 1326
-
3.2.1.55 Streptomyces thermoviolaceus
-
 OPC-520
-
3.2.1.55 Streptomyces thermoviolaceus OPC-520
-
 OPC-520
-
3.2.1.55 Thermoclostridium stercorarium
-
-
-
3.2.1.55 Trichoderma reesei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
 Hordeum vulgare
3.2.1.55
-
 Streptomyces chartreusis
3.2.1.55 recombinant enzyme, expressed in Escherichia coli Streptomyces thermoviolaceus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 L-arabinoxylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Hordeum vulgare L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Aspergillus sojae L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Trichoderma reesei L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Streptomyces lividans L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Thermoclostridium stercorarium L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Streptomyces chartreusis L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Streptomyces thermoviolaceus L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Streptomyces chartreusis GS901 L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Streptomyces thermoviolaceus OPC-520 L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Streptomyces lividans 66 1326 L-arabinose + ?
-
 ir
3.2.1.55 additional information
-
 Aspergillus niger ?
-
 ?
3.2.1.55 additional information
-
 Hordeum vulgare ?
-
 ?
3.2.1.55 additional information
-
 Aspergillus sojae ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces lividans ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces chartreusis ?
-
 ?
3.2.1.55 additional information no hydrolysis observed with birchwood xylan, linear 1,5-arabinan, arabinogalactan, gum arabic, p-nitrophenyl alpha-L-arabinopyranoside, p-nitrophenyl beta-D-xylopyranoside, p-nitrophenyl beta-D-galactopyranoside or p-nitrophenyl beta-D-glucopyranoside Streptomyces thermoviolaceus ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces chartreusis GS901 ?
-
 ?
3.2.1.55 additional information no hydrolysis observed with birchwood xylan, linear 1,5-arabinan, arabinogalactan, gum arabic, p-nitrophenyl alpha-L-arabinopyranoside, p-nitrophenyl beta-D-xylopyranoside, p-nitrophenyl beta-D-galactopyranoside or p-nitrophenyl beta-D-glucopyranoside Streptomyces thermoviolaceus OPC-520 ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces lividans 66 1326 ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Aspergillus sojae L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Trichoderma reesei L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Aspergillus awamori L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Thermoclostridium stercorarium L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Streptomyces thermoviolaceus L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Streptomyces thermoviolaceus OPC-520 L-arabinose + ?
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Hordeum vulgare p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus sojae p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Trichoderma reesei p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Thermoclostridium stercorarium p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces thermoviolaceus p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis GS901 p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces thermoviolaceus OPC-520 p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans 66 1326 p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus nidulans L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Hordeum vulgare L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus sojae L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Trichoderma reesei L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus awamori L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Thermoclostridium stercorarium L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces thermoviolaceus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces chattanoogensis L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces coelicolor L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis GS901 L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces thermoviolaceus OPC-520 L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans 66 1326 L-arabinose + phenol
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 60
-
-
 Aspergillus niger
3.2.1.55 60
-
-
 Hordeum vulgare
3.2.1.55 60
-
-
 Aspergillus sojae
3.2.1.55 60
-
-
 Trichoderma reesei
3.2.1.55 60
-
-
 Streptomyces lividans
3.2.1.55 60
-
-
 Aspergillus awamori
3.2.1.55 60
-
-
 Streptomyces thermoviolaceus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 50 80 stable up to 50°C, retains 50% original activity at 80°C Streptomyces thermoviolaceus
3.2.1.55 60
-
-
 Aspergillus nidulans
3.2.1.55 60
-
-
 Hordeum vulgare
3.2.1.55 60
-
 loses activity completely after 10 min Streptomyces lividans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 5
-
-
 Aspergillus niger
3.2.1.55 5
-
-
 Aspergillus sojae
3.2.1.55 5
-
-
 Aspergillus awamori
3.2.1.55 5
-
-
 Thermoclostridium stercorarium
3.2.1.55 5
-
-
 Streptomyces thermoviolaceus