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Literature summary extracted from

  • Bezalel, L.; Shoham, Y.; Rosenberg, E.
    Characterization and delignification activity of a thermostable alpha-L-arabinofuranosidase from Bacillus stearothermophilus (1993), Appl. Microbiol. Biotechnol., 40, 57-62.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.55 paper production
-
 Aspergillus niger
3.2.1.55 paper production extraction of lignin from wood chips, essential step in the preparation of pulp fibres Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.22
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Geobacillus stearothermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.55 extracellular
-
 Geobacillus stearothermophilus
-
-
3.2.1.55 extracellular
-
 Aspergillus niger
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 52500
-
 1 * 52500 + 1 * 57500, SDS-PAGE Geobacillus stearothermophilus
3.2.1.55 57500
-
 1 * 52500 + 1 * 57500, SDS-PAGE Geobacillus stearothermophilus
3.2.1.55 108000
-
 gel filtration, Sepharose Cl 6B Geobacillus stearothermophilus
3.2.1.55 110000
-
 gel filtration Geobacillus stearothermophilus
3.2.1.55 114800
-
 gel filtration, Superose 12 Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 softwood Kraft pulp + H2O Geobacillus stearothermophilus
-
 lignin + ?
-
 ?
3.2.1.55 softwood Kraft pulp + H2O Geobacillus stearothermophilus L1
-
 lignin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aspergillus niger
-
-
-
3.2.1.55 Geobacillus stearothermophilus
-
 L1
-
3.2.1.55 Geobacillus stearothermophilus L1
-
 L1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 101
-
-
 Geobacillus stearothermophilus
3.2.1.55 126
-
 purified enzyme Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 L-arabinogalactan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ?
3.2.1.55 L-arabinogalactan + H2O low activity on the substrate Geobacillus stearothermophilus L-arabinose + ?
-
 ?
3.2.1.55 L-arabinogalactan + H2O low activity on the substrate Geobacillus stearothermophilus L1 L-arabinose + ?
-
 ?
3.2.1.55 L-arabinoxylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O low activity on the substrate Geobacillus stearothermophilus L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O low activity on the substrate Geobacillus stearothermophilus L1 L-arabinose + ?
-
 ir
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus L1 p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus L1 L-arabinose + phenol
-
 ir
3.2.1.55 softwood Kraft pulp + H2O
-
 Geobacillus stearothermophilus lignin + ?
-
 ?
3.2.1.55 softwood Kraft pulp + H2O
-
 Geobacillus stearothermophilus L1 lignin + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 dimer 1 * 52500 + 1 * 57500, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 70
-
 at pH 7 and pH 8 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 60 80 at pH 7 enzyme is completely stable at 60°C for at least 80 min, retains 50% of its maximum activity after 75 min at 70°C and loses all activity after 15 min at 80°C Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 7
-
-
 Geobacillus stearothermophilus