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Literature summary extracted from

  • Hatanaka, H.; Imaoka, H.; Tajima, S.; Kasai, T.
    Purification and properties of an alpha-L-arabinofuranosidase from cotyledons of soybean seedlings (1991), Agric. Biol. Chem., 55, 2599-2605.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.55 D-galactono-1,4-lactone 0.5 mM, relative enzyme activity 102% Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Ba2+
-
 Glycine max
3.2.1.55 Cu2+
-
 Glycine max
3.2.1.55 Cu2+
-
 Scopolia japonica
3.2.1.55 EDTA
-
 Glycine max
3.2.1.55 Hg2+
-
 Glycine max
3.2.1.55 L-arabino-gamma-lactone
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.53
-
 4-nitrophenyl alpha-L-arabinofuranoside
-
 Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.55 Ca2+ 0.5 mM CaCl2, relative enzyme activity 139% Glycine max
3.2.1.55 Co2+ 0.5 mM CoCl2, relative enzyme activity 111% Glycine max
3.2.1.55 Zn2+ activated by 0.5 mM ZnSO4, relative enzyme activity 180% Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 87000
-
 SDS-PAGE Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 beet arabinan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Glycine max
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Scopolia japonica
-
 L-arabinose + ?
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aspergillus niger
-
-
-
3.2.1.55 Daucus carota
-
 carrot
-
3.2.1.55 Glycine max
-
 soybean
-
3.2.1.55 Scopolia japonica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
 Aspergillus niger
3.2.1.55
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.55 callus
-
 Scopolia japonica
-
3.2.1.55 cotyledon
-
 Glycine max
-
3.2.1.55 seed
-
 Glycine max
-
3.2.1.55 seedling
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 95.2
-
-
 Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 1,5-L-arabinan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
3.2.1.55 1,5-L-arabinan + H2O
-
 Glycine max L-arabinose + ?
-
 ir
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Glycine max 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Glycine max L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Scopolia japonica L-arabinose + ?
-
 ir
3.2.1.55 L-arabinan + H2O
-
 Aspergillus niger (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Daucus carota (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 additional information
-
 Aspergillus niger ?
-
 ?
3.2.1.55 additional information enzyme does not degrade arabinogalactan or arabinoxylan, arabinose can not be hydrolyzed by the purified enzyme, beta-1,3 bonding arabinoside are not good substrates Glycine max ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 45
-
-
 Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 50
-
 stable up to Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 4.8
-
-
 Glycine max
3.2.1.55 4.8
-
-
 Scopolia japonica