EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.12.1.1 | sulfate | - |
Thermithiobacillus tepidarius |
EC Number | General Stability | Organism |
---|---|---|
1.8.2.2 | lability of enzyme during purification and storage at -20°C | Thermithiobacillus tepidarius |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.2.2 | 0.004 | - |
thiosulfate | with cytochrome c as electron acceptor | Thermithiobacillus tepidarius | |
1.8.2.2 | 0.11 | - |
thiosulfate | with ferricyanide as electron acceptor | Thermithiobacillus tepidarius |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.2.2 | periplasm | at least 73% of the enzyme activity is recovered in the periplasmic fraction | Thermithiobacillus tepidarius | - |
- |
3.12.1.1 | periplasm | - |
Thermithiobacillus tepidarius | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.2.2 | 45000 | - |
3 * 45000, SDS-PAGE | Thermithiobacillus tepidarius |
1.8.2.2 | 138000 | - |
gel filtration | Thermithiobacillus tepidarius |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.2.2 | thiosulfate + cytochrome c | Thermithiobacillus tepidarius | - |
tetrathionate + reduced cytochrome c | - |
? | |
3.12.1.1 | trithionate + H2O | Thermithiobacillus tepidarius | involved in cytochrome c reduction | sulfate + thiosulfate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.2.2 | Thermithiobacillus tepidarius | - |
- |
- |
3.12.1.1 | Thermithiobacillus tepidarius | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.2.2 | partial, using ion exchange chromatography, gel filtration and hydrophobic interaction chromatography | Thermithiobacillus tepidarius |
3.12.1.1 | partial | Thermithiobacillus tepidarius |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.2.2 | 1 | - |
cytoplasm | Thermithiobacillus tepidarius |
1.8.2.2 | 2 | - |
membrane | Thermithiobacillus tepidarius |
1.8.2.2 | 9.6 | - |
periplasm | Thermithiobacillus tepidarius |
1.8.2.2 | 316 | - |
partially purified enzyme | Thermithiobacillus tepidarius |
3.12.1.1 | 0.008 | 0.014 | crude extract | Thermithiobacillus tepidarius |
3.12.1.1 | 0.038 | - |
partially purified enzyme after stored for 2-3 d at 4°C | Thermithiobacillus tepidarius |
EC Number | Storage Stability | Organism |
---|---|---|
1.8.2.2 | 20-30% loss of activity after freezing at -20°C and thawing | Thermithiobacillus tepidarius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.2.2 | thiosulfate + cytochrome c | - |
Thermithiobacillus tepidarius | tetrathionate + reduced cytochrome c | - |
? | |
1.8.2.2 | thiosulfate + oxidized acceptor | ferricyanide or native cytochrome c act as electron acceptors | Thermithiobacillus tepidarius | tetrathionate + reduced acceptor | - |
? | |
1.8.2.2 | thiosulfate + oxidized acceptor | cytochrome c acts as electron acceptor | Thermithiobacillus tepidarius | tetrathionate + reduced acceptor | - |
? | |
1.8.2.2 | thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Thermithiobacillus tepidarius | tetrathionate + reduced acceptor | - |
? | |
3.12.1.1 | trithionate + H2O | - |
Thermithiobacillus tepidarius | sulfate + thiosulfate | - |
? | |
3.12.1.1 | trithionate + H2O | involved in cytochrome c reduction | Thermithiobacillus tepidarius | sulfate + thiosulfate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.2.2 | trimer | 3 * 45000, SDS-PAGE | Thermithiobacillus tepidarius |