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Literature summary extracted from

  • Ober, D.; Tholl, D.; Martin, W.; Hartmann, T.
    Homospermidine synthase of Rhodopseudomonas viridis: substrate specificity and effects of the heterologously expressed enzyme of polyamine metabolism of Escherichia coli (1996), J. Gen. Appl. Microbiol., 42, 411-420.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.44 0.001
-
 NAD+
-
 Blastochloris viridis
2.5.1.44 0.27
-
 putrescine
-
 Blastochloris viridis
2.5.1.44 1.5
-
 spermidine in presence of 1 mM putrescine Blastochloris viridis
2.5.1.44 1.7
-
 putrescine
-
 Blastochloris viridis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.44 K+ activates, optimal concentration at 50 mM Blastochloris viridis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.44 2 putrescine Blastochloris viridis
-
 sym-homospermidine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.44 Blastochloris viridis
-
 expressed in Escherichia coli strain BL21/pHsRvT72.2
-
2.5.1.44 Blastochloris viridis
-
 DSM 134
-
2.5.1.44 Blastochloris viridis
-
 accession no. L77975
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.44 culture medium
-
 Blastochloris viridis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.44 2 putrescine
-
 Blastochloris viridis sym-homospermidine + NH3
-
 ?
2.5.1.44 putrescine + 1,3-diaminopropane
-
 Blastochloris viridis homospermidine + spermidine
-
 ?
2.5.1.44 putrescine + 1,6 diaminohexane
-
 Blastochloris viridis homospermidine + N-(4-aminobutyl)-1,6-diaminohexane
-
 ?
2.5.1.44 putrescine + 1,7-diaminoheptane
-
 Blastochloris viridis homospermidine + N-(4-aminobutyl)-1,7-diaminoheptane
-
 ?
2.5.1.44 putrescine + cadaverine
-
 Blastochloris viridis homospermidine + N-(4-aminobutyl)-1,5-diaminopentane
-
 ?
2.5.1.44 spermidine
-
 Blastochloris viridis homospermidine + putrescine + 1,3-diaminopropane
-
 ?
2.5.1.44 spermidine + 1,6-diaminohexane
-
 Blastochloris viridis homospermidine + N-(4-aminobutyl)-1,6-diaminohexane
-
 ?
2.5.1.44 spermidine + cadaverine
-
 Blastochloris viridis homospermidine + N-(4-aminobutyl)-1,5-diaminopentane
-
 ?
2.5.1.44 spermidine + putrescine
-
 Blastochloris viridis homospermidine + 1,3-diaminopropane
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.44 45
-
 increasing activity up to 45°C, sharp decrease at higher temperatures Blastochloris viridis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.44 -18
-
 storage as acetone dry-powder, stable for several months Blastochloris viridis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.44 8.8 9 in 50 mM potassium phosphate or Bis-Tris-propane buffers Blastochloris viridis

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.44 NAD+
-
 Blastochloris viridis
2.5.1.44 NADP+ 68% of the activity with NAD+ Blastochloris viridis