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Literature summary extracted from

  • Paniego, N.B.; Zuurbier, K.W.M.; Fung, S.Y.; Van der Heijden, R.; Scheffer, J.J.C.; Verpoorte, R.
    Phlorisovalerophenone synthase, a novel polyketide synthase from hop (Humulus lupulus L.) cones (1999), Eur. J. Biochem., 262, 612-616.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.156 nutrition cones of the hop plant used in the beer-brewing process Humulus lupulus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.156 characterization of the VPS cDNA clone Humulus lupulus

General Stability

EC Number General Stability Organism
2.3.1.156 low stability Humulus lupulus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.156 0.004
-
 isovaleryl-CoA
-
 Humulus lupulus
2.3.1.156 0.01
-
 isobutyryl-CoA
-
 Humulus lupulus
2.3.1.156 0.033
-
 malonyl-CoA
-
 Humulus lupulus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.156 45000
-
 homodimer, 2 * 45000, SDS-PAGE Humulus lupulus
2.3.1.156 110000
-
 native PAGE of purified VPS Humulus lupulus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.156 Humulus lupulus
-
 hop
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.156
-
 Humulus lupulus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.156 isovaleryl-CoA + 3 malonyl-CoA = 4 CoA + 3 CO2 + phlorisovalerophenone Closely related to EC 2.3.1.74, chalcone synthase. The product, 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one, is chloroisovalerophenone. Also acts on isobutyryl-CoA as substrate to give phlorisobutyrophenone. The products are intermediates in the biosynthesis of the bitter alpha-acids in hops, Humulus lupulus Humulus lupulus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.156 cone glandular hairs Humulus lupulus
-
2.3.1.156 inflorescence glands Humulus lupulus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.156 isobutyryl-CoA + 3 malonyl-CoA
-
 Humulus lupulus phloroisobutyrophenone + 3 CO2 + 4 CoA
-
 ?
2.3.1.156 isovaleryl-CoA + malonyl-CoA
-
 Humulus lupulus phloroisovalerophenone + CO2 + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.156 dimer homodimer, 2 * 45000, SDS-PAGE Humulus lupulus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.156 VPS
-
 Humulus lupulus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.156 7
-
-
 Humulus lupulus