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Literature summary extracted from

  • Choi, K.B.; Morishige, T.; Sato, F.
    Purification and characterization of coclaurine N-methyltransferase from cultured Coptis japonica cells (2001), Phytochemistry, 56, 649-655.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.140 Co2+ 5 mM, 75% inhibition; 75% inhibition at 5 mM Co2+ Coptis japonica
2.1.1.140 Cu2+ 47% inhibition at 5 mM Cu2+; 5 mM, 47% inhibition Coptis japonica
2.1.1.140 Mn2+ 57% inhibition at 5 mM Mn2+; 5 mM, 57% inhibition Coptis japonica
2.1.1.140 additional information no inhibition by PCMB, iodoacetamide; not inhibited by EDTA, iodoacetamide, p-chloromercuribenzoate Coptis japonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.140 0.38
-
 (R,S)-norreticuline
-
 Coptis japonica
2.1.1.140 0.65
-
 S-adenosyl-L-methionine
-
 Coptis japonica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.140 Ca2+ 25% increase in activity at 5 mM Ca2+ Coptis japonica
2.1.1.140 Ca2+ 5 mM, 25% increase in activity Coptis japonica
2.1.1.140 additional information no metal ion requirement Coptis japonica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.140 45000
-
 4 * 45000, SDS-PAGE Coptis japonica
2.1.1.140 45000
-
 alpha, 4 * 45000, SDS-PAGE Coptis japonica
2.1.1.140 160000
-
 gel filtration Coptis japonica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.140 (S)-coclaurine + S-adenosyl-L-methionine Coptis japonica
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.140 Coptis japonica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.140
-
 Coptis japonica
2.1.1.140 30-50% ammonium sulfate, Phenyl Sepharose, Q-Sepharose, Mono Q, Mono P, 343fold purification Coptis japonica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.140 cell culture
-
 Coptis japonica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.140 0.017
-
-
 Coptis japonica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.140 (R)-coclaurine + S-adenosyl-L-methionine
-
 Coptis japonica S-adenosyl-L-homocysteine + (R)-N-methylcoclaurine
-
 ?
2.1.1.140 (R,S)-6-O-methylnorlaudanosoline + S-adenosyl-L-methionine
-
 Coptis japonica S-adenosyl-L-homocysteine + (R,S)-N-methyl-6-O-methyl-norlaudanosoline
-
 ?
2.1.1.140 (R,S)-norlaudanosoline + S-adenosyl-L-methionine
-
 Coptis japonica S-adenosyl-L-homocysteine + (R,S)-N-methylnorlaudanosoline
-
 ?
2.1.1.140 (R,S)-norreticuline + S-adenosyl-L-methionine
-
 Coptis japonica S-adenosyl-L-homocysteine + (R,S)-N-methylnorreticuline
-
 ?
2.1.1.140 (S)-coclaurine + S-adenosyl-L-methionine
-
 Coptis japonica S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
2.1.1.140 1-methyl-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline + S-adenosyl-L-methionine
-
 Coptis japonica N-methyl-1-methyl-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline + S-adenosyl-L-methionine
-
 ?
2.1.1.140 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline + S-adenosyl-L-methionine
-
 Coptis japonica N-methyl-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline + S-adenosyl-L-methionine
-
 ?
2.1.1.140 additional information no activity with (R,S)-scoulerine, 1,2,3,4-tetrahydroisoquinoline, 1,2,3,4-tetrahydro-3-isoquinolinecarboxylic acid or (+)-emetine Coptis japonica ?
-
 ?
2.1.1.140 S-adenosyl-L-methionine + (R)-coclaurine 122% of the activity with (S)-coclaurine Coptis japonica S-adenosyl-L-homocysteine + (R)-N-methylcoclaurine
-
 ?
2.1.1.140 S-adenosyl-L-methionine + (R,S)-6-O-methylnorlaudanosoline 38% of the activity with (S)-coclaurine Coptis japonica S-adenosyl-L-homocysteine + (R,S)-N-methyl-6-O-methylnorlaudanosoline
-
 ?
2.1.1.140 S-adenosyl-L-methionine + (R,S)-norlaudanosoline 48% of the activity with (S)-coclaurine Coptis japonica S-adenosyl-L-homocysteine + (R,S)-N-methylnorlaudanosoline
-
 ?
2.1.1.140 S-adenosyl-L-methionine + (R,S)-norreticuline 55% of the activity with (S)-coclaurine Coptis japonica S-adenosyl-L-homocysteine + (R,S)-N-methylnorreticuline
-
 ?
2.1.1.140 S-adenosyl-L-methionine + (S)-coclaurine sequential bi-substrate mechanism, ordered bi bi or rapid equilibrium random bi bi Coptis japonica S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
2.1.1.140 S-adenosyl-L-methionine + 1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline 10% of the activity with (S)-coclaurine Coptis japonica S-adenosyl-L-homocysteine + 6,7-dihydroxy-1,N-dimethyl-1,2,3,4-tetrahydroisoquinoline
-
 ?
2.1.1.140 S-adenosyl-L-methionine + 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline 39% of the activity with (S)-coclaurine Coptis japonica S-adenosyl-L-homocysteine + 6,7-dimethoxy-N-methyl-1,2,3,4-tetrahydroisoquinoline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.140 homotetramer alpha, 4 * 45000, SDS-PAGE Coptis japonica
2.1.1.140 tetramer 4 * 45000, SDS-PAGE Coptis japonica

Synonyms

EC Number Synonyms Comment Organism
2.1.1.140 CNMT
-
 Coptis japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.140 7
-
-
 Coptis japonica
2.1.1.140 7
-
 50% activity at pH 6.0 and 9.0 Coptis japonica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.140 6 9 about 50% of maximal activity at pH 6.0 and 9.0 Coptis japonica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.140 Coptis japonica chromatofocusing
-
 4.2