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Literature summary extracted from

  • Prior, A.; Uhrig, J.F.; Heins, L.; Wiesmann, A.; Lillig, C.H.; Stoltze, C.; Soll, J.; Schwenn, J.D.
    Structural and kinetic properties of adenylyl sulfate reductase from Catharanthus roseus cell cultures (1999), Biochim. Biophys. Acta, 1430, 25-38.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.4.9 sulfate optimal concentration between 350 and 500 mM Catharanthus roseus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.9
-
 Catharanthus roseus
1.8.4.10 expression of wild-type and mutant enzymes lacking transit sequence or transit sequence and C-terminal domain in Escherichia coli Catharanthus roseus
1.8.99.2 expression in Escherichia coli Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.9 0.0025
-
 5'-adenylylsulfate with glutathione as reductant Catharanthus roseus
1.8.4.9 0.0038
-
 5'-adenylylsulfate with DTT as reductant Catharanthus roseus
1.8.4.9 0.4
-
 DTT with 5'-adenylylsulfate as substrate Catharanthus roseus
1.8.4.9 3
-
 glutathione with 5'-adenylylsulfate as substrate Catharanthus roseus
1.8.4.10 0.0025
-
 adenosine-5'-phosphosulfate recombinant enzyme lacking transit peptide, artificial reductand glutathione Catharanthus roseus
1.8.4.10 0.0038
-
 adenosine-5'-phosphosulfate recombinant enzyme lacking transit peptide, artificial reductand dithiothreitol Catharanthus roseus
1.8.4.10 0.4
-
 reduced dithiothreitol recombinant enzyme lacking transit peptide Catharanthus roseus
1.8.4.10 3
-
 glutathione recombinant enzyme lacking transit peptide Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.9 chloroplast precursor imported in pea chloroplast in vitro Catharanthus roseus 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.9 45000
-
 mature protein without chloroplast transit peptide, calculated from cDNA sequence Catharanthus roseus
1.8.4.9 51390
-
 calculated from cDNA sequence Catharanthus roseus
1.8.4.10 51394
-
 x * 51394, preprotein, deduced from nucleotide sequence Catharanthus roseus

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.9 Catharanthus roseus
-
-
-
1.8.4.10 Catharanthus roseus Q39619
-
-
1.8.4.10 Pisum sativum
-
-
-
1.8.99.2 Catharanthus roseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.10 recombinant His-tagged enzyme, metal affinity resin Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.10 cell suspension culture
-
 Catharanthus roseus
-
1.8.99.2 cell suspension culture axenic cell suspension culture Catharanthus roseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.9 5'-adenylylsulfate + dithioerythritol
-
 Catharanthus roseus adenosine 5'-monophosphate + sulfite + oxidized dithioerythritol
-
 ?
1.8.4.9 5'-adenylylsulfate + dithiothreitol
-
 Catharanthus roseus adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol
-
 ?
1.8.4.9 5'-adenylylsulfate + glutathione
-
 Catharanthus roseus adenosine monophosphate + sulfite + oxidized glutathione
-
 ?
1.8.4.10 5'-adenylyl sulfate + glutathione enzyme contains a C-terminal thioredoxin-like PDI domain, physiological electron acceptor unknown Catharanthus roseus AMP + sulfite + glutathione disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + reduced dithiothreitol enzyme contains a C-terminal thioredoxin-like PDI domain, physiological electron acceptor unknown Catharanthus roseus AMP + sulfite + oxidized dithiothreitol
-
 ?
1.8.99.2 adenylyl sulfate + dithioerythritol
-
 Catharanthus roseus AMP + sulfite + ?
-
 ?
1.8.99.2 adenylyl sulfate + GSH
-
 Catharanthus roseus AMP + sulfite + GSSG
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.4.10 ? x * 51394, preprotein, deduced from nucleotide sequence Catharanthus roseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.9 2.1
-
 5'-adenylylsulfate with glutathione as reductant Catharanthus roseus
1.8.4.9 3.4
-
 5'-adenylylsulfate with DTT as reductant Catharanthus roseus
1.8.4.10 2.1
-
 adenosine-5'-phosphosulfate recombinant enzyme lacking transit peptide, artificial reductant glutathione Catharanthus roseus
1.8.4.10 3.4
-
 adenosine-5'-phosphosulfate recombinant enzyme lacking transit peptide, artificial reductant dithiothreitol Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.9 8.8
-
 recombinant protein, expressed in Escherichia coli Catharanthus roseus