Any feedback?
Literature summary extracted from
- Structural analysis of the FMN binding domain of NADPH-cytochrome P-450 oxidoreductase by site-directed mutagenesis (1989), J. Biol. Chem., 264, 7584-7589.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.6.2.4 | expression in Escherichia coli | Rattus norvegicus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.6.2.4 | Y140D | substitution does not eliminate FMN binding but reduces cytochrome c reductase activity, Km value for cytochrome c or NADPH similar to wild type | Rattus norvegicus |
| 1.6.2.4 | Y140D/178D | substitution abolishes FMN binding and cytochrome c reductase activity | Rattus norvegicus |
| 1.6.2.4 | Y140F | substitution has no effect on FMN content or catalytic activity, Km value for cytochrome c or NADPH similar to wild type | Rattus norvegicus |
| 1.6.2.4 | Y140F/178F | substitution has no effect on FMN content or catalytic activity, slightly decreases Km for cytochrome c, NADPH Km value slightly higher than wild type | Rattus norvegicus |
| 1.6.2.4 | Y178D | substitution abolishes FMN binding and cytochrome c reductase activity, Km value for cytochrome c similar to wild type, NADPH Km value slightly higher than wild type | Rattus norvegicus |
| 1.6.2.4 | Y178F | substitution has no effect on FMN content or catalytic activity, slightly decreases Km for cytochrome c, NADPH Km value similar to wild type | Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.2.4 | 0.0064 | - |
NADPH | wild type, cosubstrate cytochrome c | Rattus norvegicus |  |
| 1.6.2.4 | 0.0066 | - |
NADPH | native enzyme, liver, cosubstrate cytochrome c | Rattus norvegicus | |
| 1.6.2.4 | 0.0066 | - |
NADPH | Y140D mutant, cosubstrate cytochrome c | Rattus norvegicus | |
| 1.6.2.4 | 0.0071 | - |
NADPH | Y178F mutant, cosubstrate cytochrome c | Rattus norvegicus | |
| 1.6.2.4 | 0.0078 | - |
NADPH | Y140F mutant, cosubstrate cytochrome c | Rattus norvegicus | |
| 1.6.2.4 | 0.0095 | - |
NADPH | Y178D mutant, cosubstrate cytochrome c | Rattus norvegicus | |
| 1.6.2.4 | 0.0114 | - |
NADPH | Y140F/178F double mutant, cosubstrate cytochrome c | Rattus norvegicus | |
| 1.6.2.4 | 0.0131 | - |
cytochrome c | Y140F/178F double mutant | Rattus norvegicus | |
| 1.6.2.4 | 0.014 | - |
cytochrome c | Y178F mutant | Rattus norvegicus | |
| 1.6.2.4 | 0.0178 | - |
cytochrome c | native enzyme, liver | Rattus norvegicus | |
| 1.6.2.4 | 0.0185 | - |
cytochrome c | Y178D mutant | Rattus norvegicus | |
| 1.6.2.4 | 0.0188 | - |
cytochrome c | Y140F mutant | Rattus norvegicus | |
| 1.6.2.4 | 0.0191 | - |
cytochrome c | Y140D mutant | Rattus norvegicus | |
| 1.6.2.4 | 0.0211 | - |
cytochrome c | wild type | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.4 | 80000 | - |
wild type, x * 80000, SDS-PAGE | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.2.4 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.6.2.4 | expressed in Escherichia coli | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.6.2.4 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.4 | 0.28 | - |
Y140D/178D double mutant, electron acceptor: cytochrome c | Rattus norvegicus |
| 1.6.2.4 | 0.44 | - |
Y178D mutant, electron acceptor: cytochrome c | Rattus norvegicus |
| 1.6.2.4 | 11 | - |
Y140D mutant, electron acceptor: cytochrome c | Rattus norvegicus |
| 1.6.2.4 | 46 | - |
Y140F/178F double mutant, electron acceptor: cytochrome c | Rattus norvegicus |
| 1.6.2.4 | 47.4 | - |
Y178F mutant, electron acceptor: cytochrome c | Rattus norvegicus |
| 1.6.2.4 | 48.1 | - |
Y178D mutant, electron acceptor: ferricyanide | Rattus norvegicus |
| 1.6.2.4 | 51 | - |
Y140D/178D double mutant, electron acceptor: ferricyanide | Rattus norvegicus |
| 1.6.2.4 | 51.5 | - |
bacterially expressed reductase protein, electron acceptor: cytochrome c | Rattus norvegicus |
| 1.6.2.4 | 52.9 | - |
electron acceptor: ferricyanide | Rattus norvegicus |
| 1.6.2.4 | 53.3 | - |
electron acceptor: cytochrome c | Rattus norvegicus |
| 1.6.2.4 | 55.2 | - |
Y140F mutant, electron acceptor: cytochrome c | Rattus norvegicus |
| 1.6.2.4 | 94.2 | - |
Y140F/178F double mutant, electron acceptor: ferricyanide | Rattus norvegicus |
| 1.6.2.4 | 94.8 | - |
Y140F mutant, electron acceptor: ferricyanide | Rattus norvegicus |
| 1.6.2.4 | 102 | - |
bacterially expressed reductase protein, electron acceptor: ferricyanide | Rattus norvegicus |
| 1.6.2.4 | 102 | - |
Y140D mutant, electron acceptor: ferricyanide | Rattus norvegicus |
| 1.6.2.4 | 113 | - |
Y178F mutant, electron acceptor: ferricyanide | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Rattus norvegicus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.6.2.4 | ? | wild type, x * 80000, SDS-PAGE | Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.4 | FAD | - |
Rattus norvegicus | |
| 1.6.2.4 | FMN | - |
Rattus norvegicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
