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Literature summary extracted from

  • Omura, T.; Takesue, S.
    A new method for simultaneous purification of cytochrome b5 and NADPH-cytochrome c reductase from rat liver microsomes (1970), J. Biochem., 67, 249-257.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 microsome
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Rattus norvegicus
-
 Sprague-Dawley strain
-
1.6.2.4 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 trypsin digestion followed by Sephadex filtration and column chromatography on DEAE-cellulose Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.4 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 0.025
-
 electron donor: NADH, electron acceptor: dichlorophenolindophenol Rattus norvegicus
1.6.2.4 0.03
-
 electron donor: NADPH, electron acceptor: cytochrome b5 Rattus norvegicus
1.6.2.4 0.055
-
 electron donor: NADH, electron acceptor: cytochrome c Rattus norvegicus
1.6.2.4 17.9
-
 electron donor: NADPH, electron acceptor: dichlorophenolindophenol Rattus norvegicus
1.6.2.4 37.6
-
 purified enzyme Rattus norvegicus
1.6.2.4 40.5
-
 electron donor: NADPH, electron acceptor: ferricyanide Rattus norvegicus
1.6.2.4 41.2
-
 electron donor: NADPH, electron acceptor: cytochrome c Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.6.2.4 -15°C or -20°C, 10 mM phosphate buffer, pH 7.5, several months Rattus norvegicus
1.6.2.4 0°C, some days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: cytochrome b5 Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Rattus norvegicus Sprague-Dawley 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: cytochrome b5 Rattus norvegicus Sprague-Dawley 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Rattus norvegicus Sprague-Dawley 2 ferrocytochrome c + NADP+ + H+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.2.4 25 30 diluted solutions: gradual loss of activity Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD
-
 Rattus norvegicus
1.6.2.4 NADH less effective than NADPH Rattus norvegicus
1.6.2.4 NADPH
-
 Rattus norvegicus