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Literature summary extracted from

  • Madyastha, K.M.; Coscia, C.J.
    Detergent-solubilized NADPH-cytochrome c(P-450) reductase from the higher plant, Catharanthus roseus. Purification and characterization (1979), J. Biol. Chem., 254, 2419-2427.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.2.4 additional information increasing ionic strength, increase in activity Catharanthus roseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.4 cetyltrimethyl ammonium bromide
-
Catharanthus roseus
1.6.2.4 NADP+ competitive inhibition Catharanthus roseus
1.6.2.4 p-chloromercuribenzoate
-
Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 0.0057
-
NADPH
-
Catharanthus roseus
1.6.2.4 0.0078
-
ferricytochrome c
-
Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 additional information
-
Catharanthus roseus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.6.2.4 additional information stimulation by increasing ionic strength Catharanthus roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.4 additional information
-
-
Catharanthus roseus

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Catharanthus roseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 purified 120fold by a combination of ion exchange chromatography and gel filtration and 745fold by affinity chromatography on 2',5'-ADP-Sepharose 4B Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.4 seedling
-
Catharanthus roseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 0.245
-
purification by ion exchange chromatography and gel filtration, electron acceptor: dichlorophenolindophenol Catharanthus roseus
1.6.2.4 1.12
-
purification by ion exchange chromatography and gel filtration, electron acceptor: ferricyanide Catharanthus roseus
1.6.2.4 1.429
-
purification by ion exchange chromatography and gel filtration Catharanthus roseus
1.6.2.4 17.14
-
purification by affinity chromatography on 2',5'-ADP-Sepharose 4B Catharanthus roseus
1.6.2.4 22
-
in the presence of 0.005 mM FMN Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Catharanthus roseus 2 ferrocytochrome c + NADP+ + H+
-
?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: cytochrome P450 Catharanthus roseus 2 ferrocytochrome c + NADP+ + H+
-
?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Catharanthus roseus 2 ferrocytochrome c + NADP+ + H+
-
?
1.6.2.4 NADPH + H+ + ferricytochrome c
-
Catharanthus roseus NADP+ + ferrocytochrome c
-
r

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD
-
Catharanthus roseus
1.6.2.4 FMN
-
Catharanthus roseus
1.6.2.4 NADPH
-
Catharanthus roseus