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Literature summary extracted from

  • Bertrand, J.C.; Gilewicz, M.; Bazin, H.; Azoulay, E.
    Purified detergent-solubilized NADPH-cytochrome c (P-450) reductase from Candida tropicalis grown on alkanes (1980), Biochem. Biophys. Res. Commun., 94, 889-893.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.6.2.4 FAD, FMN necessary for stabilization during purification Candida tropicalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.4 HgCl2
-
 Candida tropicalis
1.6.2.4 p-chloromercuribenzoate
-
 Candida tropicalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.4 additional information
-
 differences in MW partially due to method of solubilization Candida tropicalis
1.6.2.4 76000
-
 x * 76000, SDS-PAGE Candida tropicalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.4 NADPH + ferricytochrome P450 Candida tropicalis part of the microsomal electron-transport chains and responsible for the transfer of reducing equivalents between NADPH or NADH and cytochrome P-450 ferrocytochrome P450 + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Candida tropicalis
-
 grown on alkanes or glycerol
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 column chromatography on phenyl-sepharose CL-4B, DEAE cellulose, hydroxylapatite and Sephadex G-150 Candida tropicalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 19
-
 electron acceptor: neotetrazolium chloride Candida tropicalis
1.6.2.4 26
-
 electron acceptor: ferricyanide Candida tropicalis
1.6.2.4 28
-
 electron acceptor: menadione Candida tropicalis
1.6.2.4 42
-
 electron acceptor: dichlorophenolindophenol Candida tropicalis
1.6.2.4 56
-
-
 Candida tropicalis

Storage Stability

EC Number Storage Stability Organism
1.6.2.4 -70°C, 0.15 mM potassium phosphate buffer, pH 7, 1 mM mercaptoethanol, 1 mM EDTA, 1 micromol FMN, 1 micromol FAD, 0.3% Mulgofen BC-720, 30% glycerol, several months Candida tropicalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Candida tropicalis 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: neotetrazolium chloride Candida tropicalis 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: menadione Candida tropicalis 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: cytochrome P450 Candida tropicalis 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Candida tropicalis 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 NADPH + ferricytochrome P450 part of the microsomal electron-transport chains and responsible for the transfer of reducing equivalents between NADPH or NADH and cytochrome P-450 Candida tropicalis ferrocytochrome P450 + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.2.4 ? x * 76000, SDS-PAGE Candida tropicalis
1.6.2.4 More differences in subunit weight partially due to method of solubilization Candida tropicalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.4 7.8
-
-
 Candida tropicalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD tightly bound Candida tropicalis
1.6.2.4 FAD ratio FAD : FMN 1 : 1 Candida tropicalis
1.6.2.4 FMN tightly bound Candida tropicalis
1.6.2.4 FMN 1 mol per mol of enzyme Candida tropicalis
1.6.2.4 FMN ratio FAD : FMN 1 : 1 Candida tropicalis
1.6.2.4 NADPH
-
 Candida tropicalis