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Literature summary extracted from

  • Kuwahara, T.; White, R.A.; Agosin, M.
    A cytosolic FAD-containing enzyme catalyzing cytochrome c reduction in Trypanosoma cruzi. I. Purification and some properties (1985), Arch. Biochem. Biophys., 239, 18-28.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.2.4 Phenobarbital stimulation Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 additional information
-
 additional information
-
 Trypanosoma cruzi
1.6.2.4 0.00303
-
 NADPH
-
 Trypanosoma cruzi
1.6.2.4 0.06289
-
 ferricytochrome c
-
 Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 cytosol
-
 Trypanosoma cruzi 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.4 52000
-
 2 * 52000, SDS-PAGE Trypanosoma cruzi
1.6.2.4 100000
-
 gel filtration Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Trypanosoma cruzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 DEAE-cellulose and 2',5'-ADP-agarose column chromatography Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.4 epimastigote
-
 Trypanosoma cruzi
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 0.05
-
 cholate and Lubrol Px Trypanosoma cruzi
1.6.2.4 0.059
-
 no detergent Trypanosoma cruzi

Storage Stability

EC Number Storage Stability Organism
1.6.2.4 -90°C or -20°C, 5-10% loss of activity, reactivation by FAD, 24 h Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Trypanosoma cruzi 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: menadione Trypanosoma cruzi 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Trypanosoma cruzi 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 ferricyanide + NADPH + H+
-
 Trypanosoma cruzi ferrocyanide + NADP+
-
 r
1.6.2.4 ferricytochrome c + NADPH + H+
-
 Trypanosoma cruzi ferrocytochrome c + NADP+
-
 r
1.6.2.4 NADPH + H+ + menadione
-
 Trypanosoma cruzi NADP+ + menadiol
-
 r
1.6.2.4 NADPH + H+ oxidized 2,6-dichlorophenolindophenol
-
 Trypanosoma cruzi ferrocytochrome c + reduced 2,6-dichlorophenolindophenol
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.2.4 dimer 2 * 52000, SDS-PAGE Trypanosoma cruzi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.2.4 -90
-
 inactivation Trypanosoma cruzi
1.6.2.4 2 4 1 day, inactivation Trypanosoma cruzi
1.6.2.4 20
-
 1 day, from 5% to 10% loss of activity Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.4 1.45
-
 menadione
-
 Trypanosoma cruzi
1.6.2.4 7.63
-
 oxidized 2,6-dichlorophenolindophenol
-
 Trypanosoma cruzi
1.6.2.4 15
-
 ferricytochrome c
-
 Trypanosoma cruzi
1.6.2.4 64.5
-
 ferricyanide
-
 Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD 1 mol per subunit Trypanosoma cruzi
1.6.2.4 NADPH
-
 Trypanosoma cruzi