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Literature summary extracted from

  • Patel, T.R.; Jure, K.G.; Jones, G.A.
    Catabolism of phloroglucinol by the rumen anaerobe Coprococcus (1981), Appl. Environ. Microbiol., 42, 1010 - 1017.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.57 2-mercaptoethanol
-
Coprococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.57 8-hydroxyquinoline enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 83% inhibition Coprococcus sp.
1.3.1.57 Co2+
-
Coprococcus sp.
1.3.1.57 Cu2+
-
Coprococcus sp.
1.3.1.57 EDTA enzyme preincubated with the inhibitor, 0.00333 mM, for 60 min at room temperature before the addition of the substrate, 11% inhibition Coprococcus sp.
1.3.1.57 Hg2+
-
Coprococcus sp.
1.3.1.57 hydroxylamine enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 9% inhibition Coprococcus sp.
1.3.1.57 iodacetate enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 21% inhibition Coprococcus sp.
1.3.1.57 Mg2+
-
Coprococcus sp.
1.3.1.57 Mn2+
-
Coprococcus sp.
1.3.1.57 NAD+ with 0.33 mM, 15% inhibition Coprococcus sp.
1.3.1.57 NADH with 0.33 mM, 11% inhibition Coprococcus sp.
1.3.1.57 NADP+ with 0.33 mM, 38% inhibition Coprococcus sp.
1.3.1.57 o-phenanthroline enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 71% inhibition Coprococcus sp.
1.3.1.57 p-chloromercuribenzoate enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 48% inhibition Coprococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.57 0.00003
-
NADPH
-
Coprococcus sp.
1.3.1.57 0.00003
-
phloroglucinol
-
Coprococcus sp.
1.3.1.57 0.00029
-
phloroglucinol
-
Coprococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.57 130000
-
gel filtration Coprococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.57 phloroglucinol + NADPH Coprococcus sp. reverse reaction about 36fold slower then that of the forward reaction dihydrophloroglucinol + NADP
-
r
1.3.1.57 phloroglucinol + NADPH Coprococcus sp. Pe5 reverse reaction about 36fold slower then that of the forward reaction dihydrophloroglucinol + NADP
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.57 Coprococcus sp.
-
-
-
1.3.1.57 Coprococcus sp. Pe5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.57 protamine sulfate precipitation, ammonium sulfate precipitation, centrifugation steps, dialysis, gel filtration Coprococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.57 phloroglucinol + NADPH
-
Coprococcus sp. dihydrophloroglucinol + NADP+
-
r
1.3.1.57 phloroglucinol + NADPH
-
Coprococcus sp. Pe5 dihydrophloroglucinol + NADP+
-
r
1.3.1.57 phloroglucinol + NADPH reverse reaction about 36fold slower then that of the forward reaction Coprococcus sp. dihydrophloroglucinol + NADP
-
r
1.3.1.57 phloroglucinol + NADPH reverse reaction about 36fold slower then that of the forward reaction Coprococcus sp. Pe5 dihydrophloroglucinol + NADP
-
r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.57 additional information
-
a rapid decay of the enzyme activity in water bath at 53°C, less then 10% of the initial activity remains after 2.5 min Coprococcus sp.
1.3.1.57 4
-
crude enzyme shows a loss activity over a 5-day period, in presence of 1 mM of either 2-mercaptoethanol, dithiothreitol, or glutathione, or combinations of these reagents, is protected from the loss of enzyme activity Coprococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.57 7.4
-
-
Coprococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.57 NADPH
-
Coprococcus sp.