EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.57 | 2-mercaptoethanol | - |
Coprococcus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.57 | 8-hydroxyquinoline | enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 83% inhibition | Coprococcus sp. | |
1.3.1.57 | Co2+ | - |
Coprococcus sp. | |
1.3.1.57 | Cu2+ | - |
Coprococcus sp. | |
1.3.1.57 | EDTA | enzyme preincubated with the inhibitor, 0.00333 mM, for 60 min at room temperature before the addition of the substrate, 11% inhibition | Coprococcus sp. | |
1.3.1.57 | Hg2+ | - |
Coprococcus sp. | |
1.3.1.57 | hydroxylamine | enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 9% inhibition | Coprococcus sp. | |
1.3.1.57 | iodacetate | enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 21% inhibition | Coprococcus sp. | |
1.3.1.57 | Mg2+ | - |
Coprococcus sp. | |
1.3.1.57 | Mn2+ | - |
Coprococcus sp. | |
1.3.1.57 | NAD+ | with 0.33 mM, 15% inhibition | Coprococcus sp. | |
1.3.1.57 | NADH | with 0.33 mM, 11% inhibition | Coprococcus sp. | |
1.3.1.57 | NADP+ | with 0.33 mM, 38% inhibition | Coprococcus sp. | |
1.3.1.57 | o-phenanthroline | enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 71% inhibition | Coprococcus sp. | |
1.3.1.57 | p-chloromercuribenzoate | enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 48% inhibition | Coprococcus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.57 | 0.00003 | - |
NADPH | - |
Coprococcus sp. | |
1.3.1.57 | 0.00003 | - |
phloroglucinol | - |
Coprococcus sp. | |
1.3.1.57 | 0.00029 | - |
phloroglucinol | - |
Coprococcus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.57 | 130000 | - |
gel filtration | Coprococcus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.57 | phloroglucinol + NADPH | Coprococcus sp. | reverse reaction about 36fold slower then that of the forward reaction | dihydrophloroglucinol + NADP | - |
r | |
1.3.1.57 | phloroglucinol + NADPH | Coprococcus sp. Pe5 | reverse reaction about 36fold slower then that of the forward reaction | dihydrophloroglucinol + NADP | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.57 | Coprococcus sp. | - |
- |
- |
1.3.1.57 | Coprococcus sp. Pe5 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.57 | protamine sulfate precipitation, ammonium sulfate precipitation, centrifugation steps, dialysis, gel filtration | Coprococcus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.57 | phloroglucinol + NADPH | - |
Coprococcus sp. | dihydrophloroglucinol + NADP+ | - |
r | |
1.3.1.57 | phloroglucinol + NADPH | - |
Coprococcus sp. Pe5 | dihydrophloroglucinol + NADP+ | - |
r | |
1.3.1.57 | phloroglucinol + NADPH | reverse reaction about 36fold slower then that of the forward reaction | Coprococcus sp. | dihydrophloroglucinol + NADP | - |
r | |
1.3.1.57 | phloroglucinol + NADPH | reverse reaction about 36fold slower then that of the forward reaction | Coprococcus sp. Pe5 | dihydrophloroglucinol + NADP | - |
r |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.1.57 | additional information | - |
a rapid decay of the enzyme activity in water bath at 53°C, less then 10% of the initial activity remains after 2.5 min | Coprococcus sp. |
1.3.1.57 | 4 | - |
crude enzyme shows a loss activity over a 5-day period, in presence of 1 mM of either 2-mercaptoethanol, dithiothreitol, or glutathione, or combinations of these reagents, is protected from the loss of enzyme activity | Coprococcus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.57 | 7.4 | - |
- |
Coprococcus sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.57 | NADPH | - |
Coprococcus sp. |