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Literature summary extracted from

  • Dörner, E.; Boll, M.
    Properties of 2-oxoglutarate:ferredoxin oxidoreductase from Thauera aromatica and its role in enzymatic reduction of the aromatic ring (2002), J. Bacteriol., 184, 3975-3983.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.2.7.3 0.5 mM KCl 0.5 M, 10% glycerol 1 mM dithiothreitol, stabilize Thauera aromatica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.3 0.01
-
oxidized ferredoxin pH 7.8, 30°C Thauera aromatica
1.2.7.3 0.11
-
2-oxoglutarate pH 7.8, 30°C Thauera aromatica
1.2.7.3 0.29
-
CoA pH 7.8, 30°C Thauera aromatica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.7.3 cytoplasm
-
Thauera aromatica 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.3 Iron-sulfur cluster two [4Fe-4S] clusters per ferredoxin, 8.3 mol iron per mol of enzyme, 7.2 mol acid-labile sulfide per mol of enzyme Thauera aromatica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.3 34000
-
2 * 66000 + 2 * 34000, alpha2-beta2, SDS-PAGE Thauera aromatica
1.2.7.3 66000
-
2 * 66000 + 2 * 34000, alpha2-beta2, SDS-PAGE Thauera aromatica
1.2.7.3 200000
-
gel filtration Thauera aromatica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.3 succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ Thauera aromatica it is proposed that the enzyme couples the complete acetyl-CoA oxidation to aromatic ring reduction by the use of the low-potential electron shuttle ferredoxin 2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.3 Thauera aromatica O87870 and Q8RJQ9 O87870: alpha-subunit, Q8RJQ9: beta-subunit
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.3 aerobic conditions, 4°C, 50% loss of activity after 1 day Thauera aromatica

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.3
-
Thauera aromatica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.7.3 additional information activity is increased 10fold to 50fold in Thauera aromatica cells grown under denitrifying conditions on an aromatic substrate compared to that of cells grown on nonaromatic substrates Thauera aromatica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.3 4.8
-
pH 7.8, 30°C Thauera aromatica

Storage Stability

EC Number Storage Stability Organism
1.2.7.3 4°C, anaerobic conditions, 100% N2-atmosphere, 4 mM MgCl2, 1 mM dithiothreitol, 2 mM thiamine diphosphate, 10% glycerol, stable for at least 1 day Thauera aromatica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.3 2-oxoglutarate + CoA + 2 oxidized ferredoxin specific for 2-oxoglutarate Thauera aromatica succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
?
1.2.7.3 succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ it is proposed that the enzyme couples the complete acetyl-CoA oxidation to aromatic ring reduction by the use of the low-potential electron shuttle ferredoxin Thauera aromatica 2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
?

Subunits

EC Number Subunits Comment Organism
1.2.7.3 More comparison of subunit structure, molecularly and phylogenetically, of several 2-ketoacid: acceptor oxidoreductases Thauera aromatica
1.2.7.3 tetramer 2 * 66000 + 2 * 34000, alpha2-beta2, SDS-PAGE Thauera aromatica

Synonyms

EC Number Synonyms Comment Organism
1.2.7.3 2-oxoglutarate:ferredoxin oxidoreductase
-
Thauera aromatica
1.2.7.3 KGOR
-
Thauera aromatica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.7.3 17
-
2-oxoglutarate pH 7.8, 30°C Thauera aromatica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.3 8.3
-
glycylglycine buffer Thauera aromatica

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.3 additional information comparison of cofactor binding motifs of 2-ketoacid:acceptor oxidoreductases Thauera aromatica
1.2.7.3 thiamine diphosphate 1.6 mol per mol of enzyme Thauera aromatica

Expression

EC Number Organism Comment Expression
1.2.7.3 Thauera aromatica inducible by growth on aromatic substrates and under denitrifiying conditions 10 to 50fold up