Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mai, X.; Adams, M.W.W.
    Characterization of a fourth type of 2-keto acid-oxidizing enzyme from a hyperthermophilic archaeon: 2-ketoglutarate ferredoxin oxidoreductase from Thermococcus litoralis (1996), J. Bacteriol., 178, 5890-5896.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.3 thiamine diphosphate 0.9 mol per mol of enzyme, stimulates but is not essential, Km: 0.009 mM Thermococcus litoralis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.3 CuSO4 0.05 mM, complete inhibition Thermococcus litoralis
1.2.7.3 additional information no inhibition: CO, no inhibition at 5 mM: sodium benzoate, NaF, sodium hypophosphite, NaN3, KCN, 2-oxohexanoate, pyruvate, 2-oxobutyrate Thermococcus litoralis
1.2.7.3 Sodium nitrite high concentrations Thermococcus litoralis
1.2.7.3 ZnCl2 0.1 mM, complete inhibition Thermococcus litoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.3 0.008
-
 oxidized ferredoxin pH 8.4, 80°C, ferredoxin from Pyrococcus furiosus Thermococcus litoralis
1.2.7.3 0.04
-
 CoA pH 8.4, 80°C Thermococcus litoralis
1.2.7.3 0.25
-
 2-oxoglutarate pH 8.4, 80°C Thermococcus litoralis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.7.3 cytoplasm
-
 Thermococcus litoralis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.3 Iron-sulfur cluster contains at least four [4Fe-4S] clusters per mol of holoenzyme Thermococcus litoralis
1.2.7.3 Mg2+ stimulates, 2.5 mM Thermococcus litoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.3 10000
-
 2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE Thermococcus litoralis
1.2.7.3 23000
-
 2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE Thermococcus litoralis
1.2.7.3 29000
-
 2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE Thermococcus litoralis
1.2.7.3 43000
-
 2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE Thermococcus litoralis
1.2.7.3 210000
-
 native PAGE Thermococcus litoralis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.3 succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ Thermococcus litoralis enzyme plays a role in synthesis, not in energy conservation 2-oxoglutarate + CoA + oxidized ferredoxin
-
 ?
1.2.7.3 succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ Thermococcus litoralis DSM 5473 enzyme plays a role in synthesis, not in energy conservation 2-oxoglutarate + CoA + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.3 Thermococcus litoralis
-
-
-
1.2.7.3 Thermococcus litoralis DSM 5473
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.3 oxygen sensitive, half-life of about 5 min Thermococcus litoralis

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.3
-
 Thermococcus litoralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.3 0.15
-
 crude extract, pH 8.4, 80°C Thermococcus litoralis
1.2.7.3 22.3
-
 pH 8.4, 80°C, purified enzyme Thermococcus litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.3 2-oxoglutarate + CoA + 2 oxidized ferredoxin pure enzyme does not utilize other 2-keto acids, such as pyruvate, phenylglyoxylate, indolepyruvate, 2-oxobutanoate or 2-oxoisovalerate, as substrates Thermococcus litoralis succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.3 2-oxoglutarate + CoA + 2 oxidized ferredoxin pure enzyme does not utilize other 2-keto acids, such as pyruvate, phenylglyoxylate, indolepyruvate, 2-oxobutanoate or 2-oxoisovalerate, as substrates Thermococcus litoralis DSM 5473 succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.3 succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ enzyme plays a role in synthesis, not in energy conservation Thermococcus litoralis 2-oxoglutarate + CoA + oxidized ferredoxin
-
 ?
1.2.7.3 succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ enzyme plays a role in synthesis, not in energy conservation Thermococcus litoralis DSM 5473 2-oxoglutarate + CoA + oxidized ferredoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.3 octamer 2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE Thermococcus litoralis

Synonyms

EC Number Synonyms Comment Organism
1.2.7.3 CoA-dependent 2-ketoglutarate oxidoreductase
-
 Thermococcus litoralis
1.2.7.3 KGOR
-
 Thermococcus litoralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.3 90
-
 above, at pH 8.0 Thermococcus litoralis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.7.3 70 95 inactive below 25°C, drastical increase of activity at 70°C Thermococcus litoralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.3 80
-
 half-life under anaerobic conditions: about 2 days, 2 mM dithionite, 2 mM dithiothreitol, pH 8.0 Thermococcus litoralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.3 9
-
-
 Thermococcus litoralis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.7.3 7 9.5 50% activity at pH 7.0 and pH 9.5, inactive at pH 5.0 and pH 10.0 Thermococcus litoralis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.7.3 5
-
 Sodium nitrite pH 8.4, 80°C Thermococcus litoralis