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Literature summary extracted from

  • Townson, S.M.; Upcroft, J.A.; Upcroft, P.
    Characterization and purification of pyruvate:ferredoxin oxidoreductase from Giardia duodenalis (1996), Mol. Biochem. Parasitol., 79, 183-193.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.1 280000
-
 dimer, non-denaturing gradient gel in absence of reducing agent Giardia intestinalis
1.2.7.1 545000
-
 tetramer, non-denaturing gradient gel in absence of reducing agent Giardia intestinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin Giardia intestinalis
-
 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin Giardia intestinalis BRIS/83/HEPU/106
-
 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.1 Giardia intestinalis
-
-
-
1.2.7.1 Giardia intestinalis BRIS/83/HEPU/106
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.1
-
 Giardia intestinalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.1 40
-
 pH 7.5, 25°C Giardia intestinalis

Storage Stability

EC Number Storage Stability Organism
1.2.7.1 -70°C, anaerobically in presence of CoASH, thiamine diphosphate anfd Mg2+, enzyme concentration below 0.02 mg/ml, stable for months Giardia intestinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 2-oxobutyrate + CoA + oxidized methyl viologen the enzyme reacts with oxaloacetate at 4.8 activity relative to the activity against pyruvate Giardia intestinalis propanoyl-CoA + CO2 + reduced methyl viologen
-
 ?
1.2.7.1 2-oxobutyrate + CoA + oxidized methyl viologen the enzyme reacts with oxaloacetate at 4.8 activity relative to the activity against pyruvate Giardia intestinalis BRIS/83/HEPU/106 propanoyl-CoA + CO2 + reduced methyl viologen
-
 ?
1.2.7.1 additional information no activity with 2-oxoglutarate, oxomalonate, 2-oxoisocaproate, phosphoenolpyruvate, indole-3-pyruvate, or phenylpyruvate Giardia intestinalis ?
-
 ?
1.2.7.1 additional information no activity with 2-oxoglutarate, oxomalonate, 2-oxoisocaproate, phosphoenolpyruvate, indole-3-pyruvate, or phenylpyruvate Giardia intestinalis BRIS/83/HEPU/106 ?
-
 ?
1.2.7.1 oxaloacetate + CoA + oxidized methyl viologen the enzyme reacts with oxaloacetate at 40% activity relative to the activity against pyruvate Giardia intestinalis ?
-
 ?
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin
-
 Giardia intestinalis acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin no activity with NAD+ or NADP+ as electron aceptor Giardia intestinalis acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin
-
 Giardia intestinalis BRIS/83/HEPU/106 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin no activity with NAD+ or NADP+ as electron aceptor Giardia intestinalis BRIS/83/HEPU/106 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized methyl viologen
-
 Giardia intestinalis acetyl-CoA + CO2 + reduced methyl viologen
-
 ?
1.2.7.1 pyruvate + CoA + oxidized methyl viologen
-
 Giardia intestinalis BRIS/83/HEPU/106 acetyl-CoA + CO2 + reduced methyl viologen
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.7.1 PFOR
-
 Giardia intestinalis
1.2.7.1 pyruvate:ferredoxin oxidoreductase
-
 Giardia intestinalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.1 55
-
 1 h, stable Giardia intestinalis