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Literature summary extracted from

  • Yoon, K.S.; Ishii, M.; Kodama, T.; Igarashi, Y.
    Purification and characterization of pyruvate:ferredoxin oxidoreductase from Hydrogenobacter thermophilus TK-6 (1997), Arch. Microbiol., 167, 275-279.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.1 0.054
-
 CoA pH 7.6, 70°C Hydrogenobacter thermophilus
1.2.7.1 3.45
-
 pyruvate pH 7.6, 70°C Hydrogenobacter thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.1 24500
-
 1 * 46000 + 1 * 31500 + 1 * 29000 + 1 * 24500, SDS-PAGE Hydrogenobacter thermophilus
1.2.7.1 29000
-
 1 * 46000 + 1 * 31500 + 1 * 29000 + 1 * 24500, SDS-PAGE Hydrogenobacter thermophilus
1.2.7.1 31500
-
 1 * 46000 + 1 * 31500 + 1 * 29000 + 1 * 24500, SDS-PAGE Hydrogenobacter thermophilus
1.2.7.1 46000
-
 1 * 46000 + 1 * 31500 + 1 * 29000 + 1 * 24500, SDS-PAGE Hydrogenobacter thermophilus
1.2.7.1 135000
-
 gel filtration Hydrogenobacter thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin Hydrogenobacter thermophilus ferredoxin is an electron donor for the enzyme reaction in vivo acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin Hydrogenobacter thermophilus TK-6 / IAM 12695 ferredoxin is an electron donor for the enzyme reaction in vivo acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.1 Hydrogenobacter thermophilus
-
-
-
1.2.7.1 Hydrogenobacter thermophilus TK-6 / IAM 12695
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.1 the enzyme is inactivated by oxygen, which makes it difficult to purify the enzyme under oxic conditions. The enzyme can be stabilized when 0.1% Triton X-100 is added to the purification buffers Hydrogenobacter thermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.1
-
 Hydrogenobacter thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.1 3.46
-
 pH 7.6, 70°C Hydrogenobacter thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 2-oxobutyrate + CoA + oxidized methyl viologen the enzyme reacts with 2-oxobutyrate and oxaloacetate at 3–8% activity relative to the activity against pyruvate Hydrogenobacter thermophilus propanoyl-CoA + CO2 + reduced methyl viologen
-
 ?
1.2.7.1 2-oxobutyrate + CoA + oxidized methyl viologen the enzyme reacts with 2-oxobutyrate and oxaloacetate at 3–8% activity relative to the activity against pyruvate Hydrogenobacter thermophilus TK-6 / IAM 12695 propanoyl-CoA + CO2 + reduced methyl viologen
-
 ?
1.2.7.1 additional information no activity with 2-oxoglutarate, oxomalonate, 2-oxoisocaproate, phosphoenolpyruvate, indole-3-pyruvate, or phenylpyruvate Hydrogenobacter thermophilus ?
-
 ?
1.2.7.1 additional information no activity with 2-oxoglutarate, oxomalonate, 2-oxoisocaproate, phosphoenolpyruvate, indole-3-pyruvate, or phenylpyruvate Hydrogenobacter thermophilus TK-6 / IAM 12695 ?
-
 ?
1.2.7.1 oxaloacetate + CoA + oxidized methyl viologen the enzyme reacts with 2-oxobutyrate and oxaloacetate at 3–8% activity relative to the activity against pyruvate Hydrogenobacter thermophilus ?
-
 ?
1.2.7.1 oxaloacetate + CoA + oxidized methyl viologen the enzyme reacts with 2-oxobutyrate and oxaloacetate at 3–8% activity relative to the activity against pyruvate Hydrogenobacter thermophilus TK-6 / IAM 12695 ?
-
 ?
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin ferredoxin is an electron donor for the enzyme reaction in vivo Hydrogenobacter thermophilus acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin ferredoxin is an electron donor for the enzyme reaction in vivo Hydrogenobacter thermophilus TK-6 / IAM 12695 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized methyl viologen specific activity with methyl viologen is 10fold higher compared to the specific activity with Hydrogenobacter thermophilus ferredoxin Hydrogenobacter thermophilus acetyl-CoA + CO2 + reduced methyl viologen
-
 ?
1.2.7.1 pyruvate + CoA + oxidized methyl viologen specific activity with methyl viologen is 10fold higher compared to the specific activity with Hydrogenobacter thermophilus ferredoxin Hydrogenobacter thermophilus TK-6 / IAM 12695 acetyl-CoA + CO2 + reduced methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.1 tetramer 1 * 46000 + 1 * 31500 + 1 * 29000 + 1 * 24500, SDS-PAGE Hydrogenobacter thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.2.7.1 pyruvate:ferredoxin oxidoreductase
-
 Hydrogenobacter thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.1 80 85
-
 Hydrogenobacter thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.1 70
-
 8 h, anoxic conditions, 50% loss of activity Hydrogenobacter thermophilus
1.2.7.1 75
-
 30 min, anoxic conditions, more than 80% residual activity after heating Hydrogenobacter thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.1 7.6 7.8
-
 Hydrogenobacter thermophilus