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Literature summary extracted from

  • Satyapal; Pundir, C.S.
    Purification and properties of an oxalate oxidase from leaves of grain sorghum hybrid CSH-5 (1993), Biochim. Biophys. Acta, 1161, 1-5.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.3.4 Cu2+ Cu2+ + FMN 0.5 mM 280% activation Sorghum sp.
1.2.3.4 Cu2+ Cu2+ + diethyldithiocarbamate 0.5 mM 60% activation Sorghum sp.
1.2.3.4 Cu2+ Cu2+ + FAD 0.5 mM 250% activation Sorghum sp.
1.2.3.4 Cu2+ Cu2+ + L-cysteine 0.5 mM 46% activation Sorghum sp.
1.2.3.4 Cu2+ Cu2+ + 2-mercaptoethanol 0.5 mM 62% activation Sorghum sp.
1.2.3.4 Cu2+ Cu2+ + 8-hydroxyquinoline 0.5 mM 100% activation Sorghum sp.
1.2.3.4 CuSO4 0.5 mM concentration 200% activation Sorghum sp.
1.2.3.4 FAD 0.5 mM concentration 31% activation Sorghum sp.
1.2.3.4 FMN 0.5 mM concentration 57% activation Sorghum sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.4 2-mercaptoethanol 0.5 mM concentration 40% inhibition Sorghum sp.
1.2.3.4 8-hydroxyquinoline 0.5 mM concentration 56% activity retained Sorghum sp.
1.2.3.4 alpha,alpha'-dipyridyl 0.5 mM concentration 26% inhibition Sorghum sp.
1.2.3.4 azide 0.5 mM concentration 57% inhibition Sorghum sp.
1.2.3.4 diethyldithiocarbamate 0.5 mM concentration 64% activity retained Sorghum sp.
1.2.3.4 Dithionite 0.5 mM concentration 42% inhibition Sorghum sp.
1.2.3.4 EDTA 0.5 mM concentration 64% activity retained Sorghum sp.
1.2.3.4 Iodide 0.5 mM concentration 14% inhibition Sorghum sp.
1.2.3.4 L-cysteine 0.5 mM concentration 60% inhibition Sorghum sp.
1.2.3.4 SrCl2 slight inhibition at 0.5 mM concentration Sorghum sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.3.4 Cu2+
-
Sorghum sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.3.4 62000
-
alpha2, 2 * 62000, SDS-PAGE Sorghum sp.
1.2.3.4 120200
-
gel filtration Sorghum sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.4 Sorghum sp.
-
-
-
1.2.3.4 Sorghum sp. CSH-5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.3.4
-
Sorghum sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.3.4 leaf
-
Sorghum sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.3.4 0.39
-
H2O2 production Sorghum sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.4 oxalic acid + O2 + 2 H+
-
Sorghum sp. 2 CO2 + H2O2
-
ir
1.2.3.4 oxalic acid + O2 + 2 H+
-
Sorghum sp. CSH-5 2 CO2 + H2O2
-
ir

Subunits

EC Number Subunits Comment Organism
1.2.3.4 dimer alpha2, 2 * 62000, SDS-PAGE Sorghum sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.3.4 40
-
-
Sorghum sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.4 5
-
-
Sorghum sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.3.4 4 7 50% activity at pH 4.0 and 7.0 Sorghum sp.