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Literature summary extracted from

  • Varalakshmi, P.; Lathika, K.M.; Raghavan, K.G.; Singh, B.B.
    Altered physicochemical characteristics of polyethylene glycol linked beet stem oxalate oxidase (1995), Biotechnol. Bioeng., 46, 254-257.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.3.4 after 90 min treatment with trypsin 10% activity retained by free protein, 35% activity retained by immobilized protein on PEG1900 and 30% activity retained by immobilized protein on PEG5000 Beta vulgaris
1.2.3.4 immobilized on PEG1900 and PEG5000 Beta vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.4 azide mM concentration Beta vulgaris
1.2.3.4 Cu2+ mM concentration Beta vulgaris
1.2.3.4 glycolate mM concentration Beta vulgaris
1.2.3.4 Hg2+ mM concentration Beta vulgaris
1.2.3.4 NaCl 10 mM concentration Beta vulgaris
1.2.3.4 nitrate mM concentration Beta vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.4 0.05
-
 oxalic acid
-
 Beta vulgaris
1.2.3.4 0.06
-
 oxalic acid immobilized on PEG1900 Beta vulgaris
1.2.3.4 0.07
-
 oxalic acid immobilized on PEG5000 Beta vulgaris

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.4 Beta vulgaris
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.3.4 stem
-
 Beta vulgaris
-

Storage Stability

EC Number Storage Stability Organism
1.2.3.4 4°C, 20 days, free protein, complete loss of activity, immobilized protein on PEG1900, 60% activity retained and immobilized protein on PEG5000, 35% activity retained Beta vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.4 oxalic acid + O2 + 2 H+
-
 Beta vulgaris 2 CO2 + H2O2
-
 ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.3.4 60
-
 after 10 min wild type 30% activity, immobilized on PEG1900 55% activity and immobilized on PEG5000 70% activity Beta vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.4 4.5
-
-
 Beta vulgaris
1.2.3.4 6.5
-
 immobilized protein Beta vulgaris