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Literature summary extracted from

  • Kotsira, V.P.; Clonis, Y.D.
    Oxalate oxidase from barley roots: purification to homogeneity and study of some molecular, catalytic, and binding properties (1997), Arch. Biochem. Biophys., 340, 239-249.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.3.4 Ca(CH3COO)2 mM concentration 71% activation Hordeum vulgare
1.2.3.4 Cu(CH3COO)2 0.1 mM concentration 20% activation Hordeum vulgare
1.2.3.4 Pb(CH3COO)2 1 mM concentration 157% activation Hordeum vulgare

Application

EC Number Application Comment Organism
1.2.3.4 medicine colorimetric or UV-determination of oxalic acid in biological fluids and beverages Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.4 2-mercaptoethanol 100% inhibition at 0.01 mM concentration Hordeum vulgare
1.2.3.4 Fe(NO3)2 1 mM concentration 98% inhibition; Fe(NO3)2 + EDTA 1 mM concentration 96% inhibition Hordeum vulgare
1.2.3.4 ferrous acetate 94% inhibition at 0.01 mM concentration; ferrous acetate + EDTA 96% inhibition at 0.01 mM concentration Hordeum vulgare
1.2.3.4 KCl 14% inhibition at 1 mM concentration Hordeum vulgare
1.2.3.4 NaCl 14% inhibition at mM concentration Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.4 0.27
-
 oxalic acid
-
 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.3.4 25000
-
 alpha5, 5 * 25000, SDS-PAGE Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.4 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.3.4
-
 Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.3.4 root
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.3.4 34
-
 H2O2 production Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.4 oxalic acid + O2 + 2 H+
-
 Hordeum vulgare 2 CO2 + H2O2
-
 ir

Subunits

EC Number Subunits Comment Organism
1.2.3.4 pentamer alpha5, 5 * 25000, SDS-PAGE Hordeum vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.3.4 60
-
 10 min, 96% recovery Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.4 3.8
-
-
 Hordeum vulgare