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Literature summary extracted from
- Formate: cytochrome oxidoreductase of Desulfovibrio vulgaris (1969), J. Biochem., 66, 473-478.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.2.3 | KCN | - |
Desulfovibrio vulgaris |  |
| 1.17.2.3 | additional information | not: p-chloromercuribenzoate, idodoacetamide, sodium hypophosphite, EDTA or o-phenanthroline | Desulfovibrio vulgaris |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.2.3 | 0.0074 | - |
formate | - |
Desulfovibrio vulgaris | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.17.2.3 | additional information | - |
MW of cytochrome c-553: 6500, gel filtration | Desulfovibrio vulgaris |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.2.3 | Desulfovibrio vulgaris | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.2.3 | - |
Desulfovibrio vulgaris |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.17.2.3 | additional information | - |
- |
Desulfovibrio vulgaris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.2.3 | formate + ferricytochrome c-553 | yeast cytochrome c, ferricyanide and phenazine methosulfate can act as acceptors, not: NAD+, NADP+, FAD, FMN, cytochrome c3 | Desulfovibrio vulgaris | CO2 + ferrocytochrome c-553 | - |
ir | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.2.3 | 30 | - |
assay at | Desulfovibrio vulgaris |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.2.3 | 6 | 7 | with K3Fe(CN)6 as acceptor | Desulfovibrio vulgaris |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.2.3 | 4.5 | 10 | pH 4.5: about 40% of activity maximum, pH 10: about 10% of activity maximum | Desulfovibrio vulgaris |
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Release 2023.1 (January 2023)
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