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Literature summary extracted from

  • Marchal, S.; Branlant, G.
    Engineered nonphosphorylating gylceraldehyde 3-phosphate dehydrogenase at position 268 binds hydroxylamine and hydrazine as acyl acceptors (2001), Eur. J. Biochem., 268, 5764-5770.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.9 E268A attacking water molecule in the hydrolysis process is poorly activated, can be overcome by the nulceophiles hydrazin and hydroxylamine Streptococcus mutans
1.2.1.9 E268Q attacking water molecule in the hydrolysis process is poorly activated, can be overcome by the nulceophiles hydrazin and hydroxylamine Streptococcus mutans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.9 hydrazin protonated and neutral form, compete with the attacking water molecule for the binding site E268, inhibition mechanism Streptococcus mutans
1.2.1.9 hydroxylamine protonated and neutral form, compete with the attacking water molecule for the binding site E268, inhibition mechanism Streptococcus mutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.9 D-glyceraldehyde-3-phosphate + NADP+ Streptococcus mutans
-
 D-3-phosphoglycerate + NADPH
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.9 Streptococcus mutans
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.9 D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH + H+ two-step mechanism and kinetics Streptococcus mutans
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.9 D-glyceraldehyde-3-phosphate + NADP+
-
 Streptococcus mutans D-3-phosphoglycerate + NADPH
-
 ir
1.2.1.9 D-glyceraldehyde-3-phosphate + NADP+ two-step mechanism Streptococcus mutans D-3-phosphoglycerate + NADPH
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.9 25
-
 assay at Streptococcus mutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.9 9
-
 assay at Streptococcus mutans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.9 additional information
-
 steady-state rate constant for mutant E268Q and E268A is pH-dependent Streptococcus mutans

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.9 NADP+
-
 Streptococcus mutans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.9 4.7
-
 hydroxylamine wild-type enzyme Streptococcus mutans
1.2.1.9 6.3
-
 hydrazin wild-type enzyme Streptococcus mutans
1.2.1.9 57
-
 hydroxylamine mutant E268Q Streptococcus mutans
1.2.1.9 95
-
 hydroxylamine mutant E268A Streptococcus mutans
1.2.1.9 109
-
 hydrazin mutant E268A Streptococcus mutans
1.2.1.9 254
-
 hydrazin mutant E268Q Streptococcus mutans