EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.9 | D-3-phosphoglycerate | competitive | Spinacia oleracea | |
1.2.1.9 | Diamide | - |
Spinacia oleracea | |
1.2.1.9 | iodoacetamide | - |
Spinacia oleracea | |
1.2.1.9 | L-Glyceraldehyde 3-phosphate | - |
Spinacia oleracea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.9 | 0.017 | - |
NADP+ | - |
Spinacia oleracea | |
1.2.1.9 | 0.031 | - |
D-glyceraldehyde 3-phosphate | - |
Spinacia oleracea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.9 | 190000 | 200000 | gel filtration | Spinacia oleracea |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.9 | D-glyceraldehyde-3-phosphate + NADP+ | Spinacia oleracea | may be part of a system that is responsible for the export of photosynthetically generated NADPH from chloroplast to cytoplasm | D-3-phosphoglycerate + NADPH | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.9 | Spinacia oleracea | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.9 | - |
Spinacia oleracea |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.2.1.9 | D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH + H+ | proposed mechanism | Spinacia oleracea |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.1.9 | leaf | - |
Spinacia oleracea | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.9 | 15 | - |
- |
Spinacia oleracea |
EC Number | Storage Stability | Organism |
---|---|---|
1.2.1.9 | 0-4°C, 50 mM Tris-HCl buffer, pH 7.5, 1 mM EDTA, 10 mM mercaptoethanol, at least 6 months, or precipitated in 60% ammonium sulfate | Spinacia oleracea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.9 | D-glyceraldehyde-3-phosphate + NADP+ | absolutely specific for D-isomer | Spinacia oleracea | D-3-phosphoglycerate + NADPH | - |
ir | |
1.2.1.9 | D-glyceraldehyde-3-phosphate + NADP+ | may be part of a system that is responsible for the export of photosynthetically generated NADPH from chloroplast to cytoplasm | Spinacia oleracea | D-3-phosphoglycerate + NADPH | - |
ir |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.9 | NADP+ | absolutely specific for | Spinacia oleracea |