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Literature summary extracted from

  • Whited, G.M.; Gibson, D.T.
    Separation and partial characterization of the enzymes of the toluene-4-monooxygenase catabolic pathway in Pseudomonas mendocina KR1 (1991), J. Bacteriol., 173, 3017-3020.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.96 4-hydroxybenzaldehyde + NADP+ + H2O Pseudomonas mendocina involved in the degradation pathway of toluene 4-hydroxybenzoate + NADPH + H+
-
 ?
1.2.1.96 4-hydroxybenzaldehyde + NADP+ + H2O Pseudomonas mendocina KR1 involved in the degradation pathway of toluene 4-hydroxybenzoate + NADPH + H+
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2 Pseudomonas mendocina enzyme of the toluene-4-monooxygenase catabolic pathway ?
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2 Pseudomonas mendocina KR1 enzyme of the toluene-4-monooxygenase catabolic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.96 Pseudomonas mendocina
-
-
-
1.2.1.96 Pseudomonas mendocina KR1
-
-
-
1.14.13.2 Pseudomonas mendocina
-
 KR1
-
1.14.13.2 Pseudomonas mendocina KR1
-
 KR1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.96 partial Pseudomonas mendocina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.96 cell culture
-
 Pseudomonas mendocina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.96 0.17
-
-
 Pseudomonas mendocina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.96 4-hydroxybenzaldehyde + NADP+ + H2O involved in the degradation pathway of toluene Pseudomonas mendocina 4-hydroxybenzoate + NADPH + H+
-
 ?
1.2.1.96 4-hydroxybenzaldehyde + NADP+ + H2O involved in the degradation pathway of toluene Pseudomonas mendocina KR1 4-hydroxybenzoate + NADPH + H+
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
 Pseudomonas mendocina protocatechuate + NADP+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
 Pseudomonas mendocina KR1 protocatechuate + NADP+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2 enzyme of the toluene-4-monooxygenase catabolic pathway Pseudomonas mendocina ?
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2 enzyme of the toluene-4-monooxygenase catabolic pathway Pseudomonas mendocina KR1 ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.96 NADP+
-
 Pseudomonas mendocina
1.14.13.2 NADPH
-
 Pseudomonas mendocina