EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.9 | additional information | several mutants are created, e.g. strains with enzyme defectives for analysis of the 4-hydroxyphenylacetate pathway | Pseudomonas putida |
EC Number | General Stability | Organism |
---|---|---|
1.2.1.60 | heating at 50°C for 3 min and immediate chilling on ice abolishes enzyme activity in crude cell extracts | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | Pseudomonas putida | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | Pseudomonas putida NCIB 9865 | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.13.11.15 | 3,4-dihydroxyphenylacetate + O2 | Pseudomonas putida | induced by 3,4-dihydroxyphenylacetate | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | Pseudomonas putida | degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | Pseudomonas putida NCIB 9865 | degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.60 | Pseudomonas putida | - |
- |
- |
1.2.1.60 | Pseudomonas putida NCIB 9865 | - |
- |
- |
1.13.11.15 | Pseudomonas putida | - |
mutant strains P23XC1, P23XC5 and P23XC7, no activity in mutant strain P23X2 | - |
1.13.11.15 | Pseudomonas putida | - |
wild-type strain P23X1 | - |
1.14.14.9 | Pseudomonas putida | - |
P23X1, NCIB 9865 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.1.60 | cell culture | strict dependency for induction of enzyme synthesis of the meta-cleavage pathway by 4-hydroxyphenylacetate | Pseudomonas putida | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.60 | 0.07 | - |
- |
Pseudomonas putida |
1.14.14.9 | additional information | - |
- |
Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | Pseudomonas putida | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | Pseudomonas putida NCIB 9865 | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.13.11.15 | 3,4-dihydroxyphenylacetate + O2 | - |
Pseudomonas putida | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
1.13.11.15 | 3,4-dihydroxyphenylacetate + O2 | induced by 3,4-dihydroxyphenylacetate | Pseudomonas putida | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
1.13.11.15 | 3,4-dihydroxyphenylacetate + O2 | - |
Pseudomonas putida NCIB 9865 | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis | Pseudomonas putida | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway | Pseudomonas putida | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis | Pseudomonas putida NCIB 9865 | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway | Pseudomonas putida NCIB 9865 | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
1.14.14.9 | additional information | strict substrate specificity | Pseudomonas putida | ? | - |
? | |
1.14.14.9 | additional information | strict substrate specificity | Pseudomonas putida NCIB 9865 | ? | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.60 | NAD+ | - |
Pseudomonas putida |