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Literature summary extracted from

  • Alonso, J.M.; Garrido-Pertierra, A.
    Carboxymethylhydroxymuconic semialdehyde dehydrogenase in the 4-hydroxyphenylacetate catabolic pathway of Pseudomonas putida (1986), Biochem. Cell Biol., 64, 1288-1293.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.60 NADH competitive inhibition with respect to NAD+, Ki: 0.027 mM Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.60 0.009
-
 5-carboxymethyl-2-hydroxymuconate semialdehyde
-
 Pseudomonas putida
1.2.1.60 0.02
-
 NAD+
-
 Pseudomonas putida
1.2.1.60 0.048
-
 NAD+
-
 Pseudomonas putida
1.2.1.60 1.2
-
 NADP+
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.60 52500
-
 3 * 52500, SDS-PAGE Pseudomonas putida
1.2.1.60 200000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ Pseudomonas putida involved in the meta-cleavage pathway of 4-hydroxyphenylacetate 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.60 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.60 to homogeneity, chromatography techniques Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.60 cell culture
-
 Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.60 11
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.2.1.60 frozen, 12 months Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ involved in the meta-cleavage pathway of 4-hydroxyphenylacetate Pseudomonas putida 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+ 18% of the activity compared to reduction with NAD+ Pseudomonas putida 5-carboxymethyl-2-hydroxymuconate + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.60 tetramer 3 * 52500, SDS-PAGE Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.60 37
-
 stable for 30 min Pseudomonas putida
1.2.1.60 47
-
 complete loss of activity after 20 min Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.60 40
-
 5-carboxymethyl-2-hydroxymuconate semialdehyde
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.60 7.8
-
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.60 NAD+ strict dependency for NAD+ reduction Pseudomonas putida
1.2.1.60 NADP+ 82% lower activity compared to NAD+ Pseudomonas putida