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Literature summary extracted from

  • Ito, K.; Takahashi, M.; Yoshimoto, T.; Tsuru, D.
    Cloning and high-level expression of the glutathione-independent formaldehyde dehydrogenase gene from Pseudomonas putida (1994), J. Bacteriol., 176, 2483-2491.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.46 expressed in Escherichia coli Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.46 4-Methylpyrazole native and recombinant protein Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.46 Zinc structural zinc-liganding amino acids: Cys-97, Cys-100, Cys-103, Cys-111 Pseudomonas putida
1.2.1.46 Zinc active-site zinc-liganding amino acids: Cys-47 and His-68 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.46 42000
-
 4 * 42000, recombinant protein, expressed in Escherichia coli, SDS-PAGE, calculation from gene sequence Pseudomonas putida
1.2.1.46 150000
-
 recombinant protein, expressed in E. coli, gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.46 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.46
-
 Pseudomonas putida
1.2.1.46 recombinant protein, expressed in Escherichia coli Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.46 24.1
-
 recombinant protein, expressed in Escherichia coli Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.46 formaldehyde + NAD+ + H2O
-
 Pseudomonas putida formate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.46 tetramer 4 * 42000, recombinant protein, expressed in Escherichia coli, SDS-PAGE, calculation from gene sequence Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.46 NAD+ coenzyme binding domain: Gly-Xaa-Gly-Xaa-Xaa-Gly Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.46 0.03
-
 4-Methylpyrazole native and recombinant protein Pseudomonas putida