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Literature summary extracted from

  • Wengenmayer, H.; Ebel, J.; Grisebach, H.
    Enzymic synthesis of lignin precursors. Purification and properties of a cinnamoyl-CoA: NADPH reductase from cell suspension cultures of soybean (Glycine max) (1976), Eur. J. Biochem., 65, 529-536.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.1.44 2-mercaptoethanol: stabilizes , only stable in presence of Glycine max
1.2.1.44 ethyleneglycol, 15%, stabilizes Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.44 4-chloromercuribenzoate 0.01 mM, 50% inhibition Glycine max
1.2.1.44 CoASH product inhibition Glycine max
1.2.1.44 iodoacetamide 1 mM, 50% inhibition Glycine max
1.2.1.44 NADP+ product inhibition Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.44 0.028
-
 NADPH cosubstrate feruloyl-CoA Glycine max
1.2.1.44 0.05
-
 5-hydroxyferuloyl-CoA
-
 Glycine max
1.2.1.44 0.073
-
 feruloyl-CoA
-
 Glycine max
1.2.1.44 0.1
-
 p-Coumaroyl-CoA
-
 Glycine max
1.2.1.44 0.12
-
 NADPH cosubstrate sinapoyl-CoA Glycine max
1.2.1.44 0.2
-
 caffeoyl-CoA
-
 Glycine max
1.2.1.44 0.29
-
 NADPH cosubstrate p-coumaroyl-CoA Glycine max
1.2.1.44 0.4
-
 sinapoyl-CoA
-
 Glycine max
1.2.1.44 0.9
-
 cinnamoyl-CoA
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.44 36000
-
 1 * 36000 SDS-PAGE Glycine max
1.2.1.44 38000
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.44 Glycine max
-
 L. var. Mandarin, soybean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.44
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.44 cell culture
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.44 16.2
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
1.2.1.44 -20°C, 100 mM Tris-HCl buffer, pH 7.5, 10% ethyleneglycol, several weeks Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.44 5-hydroxyferuloyl-CoA + NADPH + H+ i.e. 4,5-dihydroxy-3-methoxycinnamoyl-CoA Glycine max 5-hydroxyferulic aldehyde + CoA + NADP+
-
 ?
1.2.1.44 caffeoyl-CoA + NADPH
-
 Glycine max caffeic aldehyde + CoA + NADP+
-
 ?
1.2.1.44 cinnamoyl-CoA + NADPH
-
 Glycine max cinnamaldehyde + CoA + NADP+
-
 ?
1.2.1.44 feruloyl-CoA + NADPH
-
 Glycine max ferulic aldehyde + CoA + NADP+
-
 ?
1.2.1.44 p-coumaroyl-CoA + NADPH
-
 Glycine max p-coumaric aldehyde + CoA + NADP+
-
 ?
1.2.1.44 sinapoyl-CoA + NADPH
-
 Glycine max sinapic aldehyde + CoA + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.44 monomer 1 * 36000 SDS-PAGE Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.44 30
-
 assay at Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.44 6 6.2
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.44 5.4 8 pH 5.4: about 10% of activity maximum, pH 8.0: about 15% of activity maximum Glycine max

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.44 7
-
 30°C, 30 min, most stable around Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.44 NADP+
-
 Glycine max