EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.10 | sulfhydryl compounds | - |
Moorella thermoacetica |
EC Number | General Stability | Organism |
---|---|---|
1.17.1.10 | glycerol stabilizes during purification | Moorella thermoacetica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.10 | cyanide | - |
Moorella thermoacetica | |
1.17.1.10 | EDTA | - |
Moorella thermoacetica | |
1.17.1.10 | FeSO4 | - |
Moorella thermoacetica | |
1.17.1.10 | hypophosphite | inhibition removed by formate | Moorella thermoacetica | |
1.17.1.10 | mercaptoethanol | - |
Moorella thermoacetica | |
1.17.1.10 | NADP+ | substrate inhibition at 5 mM or higher concentration | Moorella thermoacetica | |
1.17.1.10 | Sodium selenite | 0.01 M | Moorella thermoacetica | |
1.17.1.10 | sulfite | inhibition of activity with NADP+ but not with methyl viologen | Moorella thermoacetica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.1.10 | 0.083 | - |
formate | - |
Moorella thermoacetica | |
1.17.1.10 | 0.11 | - |
NADP+ | - |
Moorella thermoacetica | |
1.17.1.10 | 0.277 | - |
formate | cosubstrate NADP+ | Moorella thermoacetica | |
1.17.1.10 | 2.35 | - |
reduced methyl viologen | - |
Moorella thermoacetica |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.17.1.10 | soluble | - |
Moorella thermoacetica | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 270000 | 300000 | - |
Moorella thermoacetica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.1.10 | Moorella thermoacetica | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.17.1.10 | extremely oxygen-sensitive | Moorella thermoacetica |
1.17.1.10 | rapid inactivation by air, thiol-iron complexes and formate stabilize | Moorella thermoacetica |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.1.10 | - |
Moorella thermoacetica |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 34 | - |
- |
Moorella thermoacetica |
1.17.1.10 | 113 | - |
methyl viologen as electron acceptor | Moorella thermoacetica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.1.10 | CO2 + NADPH | enzyme also catalyzes: 3. oxidation of NADPH with O2 | Moorella thermoacetica | formate + NADP+ | - |
r | |
1.17.1.10 | CO2 + NADPH | also reacts with: methyl viologen, benzyl viologen | Moorella thermoacetica | formate + NADP+ | - |
r | |
1.17.1.10 | formate + reduced methyl viologen | - |
Moorella thermoacetica | CO2 + oxidized methyl viologen | - |
r |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 85 | - |
- |
Moorella thermoacetica |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 70 | - |
1 min, absence of substrate, stable | Moorella thermoacetica |
1.17.1.10 | 73 | - |
10 min, 60% loss of activity | Moorella thermoacetica |
1.17.1.10 | 76 | - |
10 min, 90% loss of activity | Moorella thermoacetica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 7 | 9.5 | - |
Moorella thermoacetica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.10 | NADP+ | - |
Moorella thermoacetica | |
1.17.1.10 | NADPH | - |
Moorella thermoacetica |