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Literature summary extracted from

  • Andreesen, J.R.; Ljungdahl, L.G.
    Nicotinamide adenine dinucleotide phosphate-dependent formate dehydrogenase from Clostridium thermoaceticum: purification and properties (1974), J. Bacteriol., 120, 6-14.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.1.10 sulfhydryl compounds
-
Moorella thermoacetica

General Stability

EC Number General Stability Organism
1.17.1.10 glycerol stabilizes during purification Moorella thermoacetica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.10 cyanide
-
Moorella thermoacetica
1.17.1.10 EDTA
-
Moorella thermoacetica
1.17.1.10 FeSO4
-
Moorella thermoacetica
1.17.1.10 hypophosphite inhibition removed by formate Moorella thermoacetica
1.17.1.10 mercaptoethanol
-
Moorella thermoacetica
1.17.1.10 NADP+ substrate inhibition at 5 mM or higher concentration Moorella thermoacetica
1.17.1.10 Sodium selenite 0.01 M Moorella thermoacetica
1.17.1.10 sulfite inhibition of activity with NADP+ but not with methyl viologen Moorella thermoacetica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.10 0.083
-
formate
-
Moorella thermoacetica
1.17.1.10 0.11
-
NADP+
-
Moorella thermoacetica
1.17.1.10 0.277
-
formate cosubstrate NADP+ Moorella thermoacetica
1.17.1.10 2.35
-
reduced methyl viologen
-
Moorella thermoacetica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.1.10 soluble
-
Moorella thermoacetica
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.10 270000 300000
-
Moorella thermoacetica

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.10 Moorella thermoacetica
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.17.1.10 extremely oxygen-sensitive Moorella thermoacetica
1.17.1.10 rapid inactivation by air, thiol-iron complexes and formate stabilize Moorella thermoacetica

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.10
-
Moorella thermoacetica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.10 34
-
-
Moorella thermoacetica
1.17.1.10 113
-
methyl viologen as electron acceptor Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.10 CO2 + NADPH enzyme also catalyzes: 3. oxidation of NADPH with O2 Moorella thermoacetica formate + NADP+
-
r
1.17.1.10 CO2 + NADPH also reacts with: methyl viologen, benzyl viologen Moorella thermoacetica formate + NADP+
-
r
1.17.1.10 formate + reduced methyl viologen
-
Moorella thermoacetica CO2 + oxidized methyl viologen
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.10 85
-
-
Moorella thermoacetica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.10 70
-
1 min, absence of substrate, stable Moorella thermoacetica
1.17.1.10 73
-
10 min, 60% loss of activity Moorella thermoacetica
1.17.1.10 76
-
10 min, 90% loss of activity Moorella thermoacetica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.10 7 9.5
-
Moorella thermoacetica

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.10 NADP+
-
Moorella thermoacetica
1.17.1.10 NADPH
-
Moorella thermoacetica