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Literature summary extracted from

  • Johnson, R.C.; Gilbertson, J.R.
    Isolation, characterization, and partial purification of a fatty acyl coenzyme A reductase from bovine cardiac muscle (1972), J. Biol. Chem., 247, 6991-6998.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.42 HgCl2
-
 Bos taurus
1.2.1.42 p-chloromercuribenzoate
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.42 85000 95000 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.42 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.42 heating, ammonium sulfate, gel filtration Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.42 hexadecanal + CoA + NAD+ = hexadecanoyl-CoA + NADH + H+ also acts, more slowly, on octadecanoyl-CoA Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.42 cardiac muscle
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.42 321
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.42 hexadecanoyl-CoA + NADH reduction of NAD+ is at most 5% of the rate of NADH oxidoreduction, the enzyme is primarily a reductase and not an aldehyde dehydrogenase Bos taurus hexadecanal + NAD+ + CoA
-
 ?
1.2.1.42 octadecanoyl-CoA + NADH slowly, octadecanoyl-CoA is identical with stearoyl-CoA Bos taurus octadecanal + NAD+ + CoA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.42 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.42 7.2
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.42 6.5 9 pH 6.5 and pH 9.0: about 20% of maximum activity Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.42 NADH
-
 Bos taurus