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Literature summary extracted from

  • Gross, G.G.
    Formation and reduction of intermediate acyladenylate by aryl-aldehyde. NADP oxidoreductase from Neurospora crassa (1972), Eur. J. Biochem., 31, 585-592.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.30 dithiothreitol stimulation of the reduction of the intermediate benzoyladenosine 5'-monophosphate to benzaldehyde Neurospora crassa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.30 AMP inhibition of the exchange reaction of diphosphate and ATP Neurospora crassa
1.2.1.30 hydroxylamine inhibition of the exchange reaction of diphosphate and ATP Neurospora crassa
1.2.1.30 iodoacetamide inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol Neurospora crassa
1.2.1.30 iodoacetate inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol Neurospora crassa
1.2.1.30 N-ethylmaleimide inhibition of the reduction of benzoyladenosine 5'-monophosphate Neurospora crassa
1.2.1.30 NADPH inhibition of the exchange reaction of diphosphate and ATP, inhibitory effect increases when dithiothreitol is added Neurospora crassa
1.2.1.30 o-Iodosobenzoate inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol Neurospora crassa
1.2.1.30 p-chloromercuribenzoate inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol Neurospora crassa
1.2.1.30 p-diazobenzenesulfonate inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.30 0.063
-
 benzoate
-
 Neurospora crassa
1.2.1.30 0.13
-
 NADPH reduction of the intermediate benzoyladenylate to benzaldehyde Neurospora crassa
1.2.1.30 0.2
-
 benzoyladenosine 5'-monophosphate
-
 Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.30 Mg2+ required Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.30 aromatic acids + NADPH + H+ + ATP Neurospora crassa
-
 aromatic aldehydes + NADP+ + AMP + diphosphate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.30 Neurospora crassa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.30 mycelium
-
 Neurospora crassa
-

Storage Stability

EC Number Storage Stability Organism
1.2.1.30 pH 7.6, 50% glycerol, 1 mM EDTA, 2.5 mM mercaptoethanol, 6 months Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.30 aromatic acids + NADPH + H+ + ATP
-
 Neurospora crassa aromatic aldehydes + NADP+ + AMP + diphosphate + H2O
-
 ?
1.2.1.30 aromatic acids + NADPH + H+ + ATP
-
 Neurospora crassa aromatic aldehydes + NADP+ + AMP + diphosphate + H2O
-
 r
1.2.1.30 benzaldehyde + NADP+ + benzoyladenosine 5'-monophosphate + phosphate
-
 Neurospora crassa benzoate + NADPH + ATP
-
 r
1.2.1.30 benzoic acid + NADPH + H+ + ATP the reaction proceeds via benzoyladenosine 5'-monophosphate which is further reduced by NADPH to benzaldehyde Neurospora crassa benzaldehyde + NADP+ + AMP + diphosphate
-
 ?
1.2.1.30 cinnamic acid + NADPH + ATP
-
 Neurospora crassa 3-phenyl-2-propen-1-al + NADP+ + AMP + H2O
-
 ?
1.2.1.30 m-hydroxybenzoic acid + NADPH + ATP
-
 Neurospora crassa m-hydroxybenzaldehyde + NADP+ + AMP + H2O
-
 ?
1.2.1.30 p-anisic acid + NADPH + ATP
-
 Neurospora crassa p-methoxybenzaldehyde + NADP+ + AMP + H2O
-
 ?
1.2.1.30 p-coumaric acid + NADPH + ATP
-
 Neurospora crassa 3-(4-hydroxphenyl)-2-propen-1-al + NADP+ + AMP + H2O
-
 ?
1.2.1.30 p-hydroxybenzoic acid + NADPH + ATP
-
 Neurospora crassa p-hydroxybenzaldehyde + NADP+ + AMP + H2O
-
 ?
1.2.1.30 salicylic acid + NADPH + ATP rate relative to benzoate 9% Neurospora crassa salicyl aldehyde + NADP+ + AMP + H2O
-
 ?
1.2.1.30 vanillic acid + NADPH + H+ + ATP
-
 Neurospora crassa 4-hydroxy-3-methoxybenzaldehyde + NADP+ + AMP + diphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.30 6.8
-
 for the reduction of benzoyladenosine 5'-monophosphate Neurospora crassa
1.2.1.30 8.4 9.2 for the exchange reaction of diphosphate and ATP Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.30 4
-
 not active below, benzoate + ATP Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.30 ATP
-
 Neurospora crassa
1.2.1.30 NADPH
-
 Neurospora crassa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.30 2.2
-
 AMP
-
 Neurospora crassa