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Literature summary extracted from

  • Willetts, A.J.; Turner, J.M.
    Threonine metabolism in a strain of Bacillus subtilis: enzymes acting on methylglyoxal (1970), Biochim. Biophys. Acta, 222, 668-670.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.23 EDTA 60% inhibition at 5 mM, 87% inhibition at 10 mM Bacillus subtilis
1.2.1.23 oxalate 60% inhibition at 5 mM, 96% inhibition at 10 mM Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.23 0.4
-
 methylglyoxal
-
 Bacillus subtilis
1.2.1.23 0.5
-
 NAD+
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.23 Ca2+ activation, 66 mM Bacillus subtilis
1.2.1.23 Mg2+ activation less effective than with Ca2+ Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.23 500000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.23 methylglyoxal + NAD+ + H2O Bacillus subtilis i.e. pyruvaldehyde pyruvate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.23 Bacillus subtilis
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.23 0.109
-
 after growth on succinate Bacillus subtilis
1.2.1.23 0.14
-
 after growth on threonine Bacillus subtilis
1.2.1.23 1.2
-
 in crude cell-free extract after growth on threonine Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.23 2-oxoaldehydes + NAD+ + H2O
-
 Bacillus subtilis 2-oxo acids + NADH
-
 ?
1.2.1.23 methylglyoxal + NAD+ + H2O i.e. pyruvaldehyde Bacillus subtilis pyruvate + NADH + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.23 9.8
-
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.23 NAD+
-
 Bacillus subtilis