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Literature summary extracted from

  • Dunkerton, J.; James, S.P.
    Some observations on the NADP+-linked oxidation of methylglyoxal catalysed by 2-Oxoaldehyde dehydrogenase (1976), Biochem. J., 153, 503-504.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.23 L-2-Aminopropan-1-ol activation of aldehyde oxidation Ovis aries
1.2.1.23 phosphate activation Ovis aries
1.2.1.49 L-2-Aminopropan-1-ol activation of aldehyde oxidation Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.23 0.11
-
 NAD+
-
 Ovis aries
1.2.1.23 2.08
-
 methylglyoxal
-
 Ovis aries
1.2.1.23 2.08
-
 methylglyoxal in the presence of L-2-aminopropan-1-ol (Km 5.0 mM), at pH 9.3 and 25°C Ovis aries
1.2.1.49 0.0045
-
 NADP+
-
 Ovis aries
1.2.1.49 0.21 0.83 methylglyoxal
-
 Ovis aries
1.2.1.49 0.83
-
 methylglyoxal
-
 Ovis aries
1.2.1.49 0.83
-
 methylglyoxal amine as activator in aldehyde oxidation Ovis aries
1.2.1.49 0.83
-
 methylglyoxal in the presence of L-2-aminopropan-1-ol (Km 83 mM), at pH 9.3 and 25°C Ovis aries

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.23 methylglyoxal + NAD+ + H2O Ovis aries i.e. pyruvaldehyde pyruvate + NADH + H+
-
 ?
1.2.1.49 methylglyoxal + NADP+ + H2O Ovis aries
-
 pyruvate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.23 Ovis aries
-
-
-
1.2.1.49 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.23 using ammonium sulfate fractionation and column chromatography on DEAE-cellulose, phosphocellulose, hydroxyapatite and Sephadex G-200 Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.23 liver
-
 Ovis aries
-
1.2.1.49 liver
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.49 0.24
-
-
 Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.23 2-oxoaldehydes + NAD+ + H2O enzyme from sheep or rat liver also active with NADP+, see also EC 1.2.1.49 Ovis aries 2-oxo acids + NADH
-
 ?
1.2.1.23 methylglyoxal + NAD+ + H2O i.e. pyruvaldehyde Ovis aries pyruvate + NADH + H+
-
 ?
1.2.1.23 additional information the presence of L-2-aminopropan-1-ol is necessary to activate the enzyme Ovis aries ?
-
 ?
1.2.1.49 2-oxoaldehydes + NADP+ + H2O enzyme from sheep or rat liver also active with NAD+, see also EC 1.2.1.23 Ovis aries 2-oxo acids + NADPH
-
 ?
1.2.1.49 methylglyoxal + NADP+ + H2O
-
 Ovis aries pyruvate + NADPH + H+
-
 ?
1.2.1.49 additional information the presence of L-2-aminopropan-1-ol is necessary to activate the enzyme Ovis aries ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.23 NAD+
-
 Ovis aries
1.2.1.23 NADP+ enzyme from sheep or rat liver, see also EC 1.2.1.49 Ovis aries
1.2.1.49 NAD+ enzyme from sheep or rat liver, see also EC 1.2.1.23 Ovis aries
1.2.1.49 NADP+
-
 Ovis aries