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Literature summary extracted from

  • Sridhara, S.; Wu, T.T.
    Purification and properties of lactaldehyde dehydrogenase from Escherichia coli (1969), J. Biol. Chem., 244, 5233-5238.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.22 cysteine
-
 Escherichia coli
1.2.1.22 glutathione
-
 Escherichia coli

General Stability

EC Number General Stability Organism
1.2.1.22 dialysis in absence of EDTA and beta-mercaptoethanol: complete loss of activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.22 Cu2+ 85% inhibition at 0.1 mM Escherichia coli
1.2.1.22 iodoacetate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.22 additional information
-
 additional information
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.22 100000
-
 sucrose density gradient centrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.22 (S)-lactaldehyde + NAD+ Escherichia coli essential for catabolism of 1,2-propanediol (S)-lactate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.22 Escherichia coli
-
 constitutive mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.22
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.22 5.1
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.22 (S)-lactaldehyde + NAD+ essential for catabolism of 1,2-propanediol Escherichia coli (S)-lactate + NADH
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O
-
 Escherichia coli (S)-lactate + NADH + H+
-
 ?
1.2.1.22 additional information specifically oxidizes L-lactaldehyde to L-lactate in presence of NAD+ Escherichia coli ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.22 10.5 11
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.22 9.2 11.2 pH 9.2: about 15% of maximum activity, pH 11.2: about 99% of maximum activity Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.22 additional information NADP+ can not substitute for NAD+ Escherichia coli
1.2.1.22 NAD+
-
 Escherichia coli