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Literature summary extracted from

  • Duarte, R.O.; Reis, A.R.; Girio, F.; Moura, I.; Moura, J.J.G.; Collaco, T.A.
    The formate dehydrogenase isolated from the aerobe Methylobacterium sp. RXM is a molybdenum-containing protein (1997), Biochem. Biophys. Res. Commun., 230, 30-34.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 Cu2+
-
 Methylobacterium sp.
1.17.1.9 imidazole
-
 Methylobacterium sp.
1.17.1.9 N3- 0.1 mM Methylobacterium sp.
1.17.1.9 PCMB
-
 Methylobacterium sp.
1.17.1.9 Zn2+
-
 Methylobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 4.7
-
 formate
-
 Methylobacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.9 Fe two distinct [Fe-S] centres Methylobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.9 75000
-
 4 * 75000, SDS-PAGE Methylobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Methylobacterium sp.
-
 RXM
-
1.17.1.9 Methylobacterium sp. RXM
-
 RXM
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.17.1.9 unstable under oxygen Methylobacterium sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.9 partial Methylobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.9 1.8
-
-
 Methylobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+
-
 Methylobacterium sp. CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+
-
 Methylobacterium sp. RXM CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.9 tetramer 4 * 75000, SDS-PAGE Methylobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.9 35
-
 10 min, stable Methylobacterium sp.
1.17.1.9 65
-
 10 min, complete inactivation Methylobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 7.5
-
-
 Methylobacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.17.1.9 6.5 10 10 min, stable Methylobacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+
-
 Methylobacterium sp.